A Comparative Investigation on Growth of Three Food Born Pathogenic Bacteria Inoculated with Withania somnifera: an Invitro Experimental Study

Authors

  • Abdoljamal Azar Department of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Sistan and Baluchistan, Iran
  • Saeed Salari Department of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Sistan and Baluchistan, Iran
  • Sedigheh Sargolzaei Department of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Sistan and Baluchistan, Iran

DOI:

https://doi.org/10.30564/vsr.v2i1.2052

Abstract

Background: Withania somnifera (WS) is proposed as one of the alternatives instead of the antibiotic. This study is aimed to evaluate the inhibitory potency of enzymatic extract of the fruits of the WS. Methods: As an invitro experimental study, the growth rate of Shigella dysenteriae, Salmonella typhimurium, and Escherichia coli inoculated in different concentrations (25%, 12.5%, 6.25% and 3.125%) of the extract were assessed. A microtitre plate method was conducted. ANOVA was applied to identify statistical differences with p-value <0.05). Compared to the microorganism control, effective concentration of the extract inhibiting the growth of E. coli was 12.5%, and 6.25%, while it was 12.5%, and 6.25% for Sh. dysenteriae (p<0.05). A dose-dependent response of E. coli was observed. The antibacterial activity of the extract tested was found mainly against E. coli and Sh. dysenteriae. The most resistant microorganism compared to E. coli and Sh. dysenteriae was S. typhimurium (p<0.05). 25% of the concentration of the extract showed the different inhibitory effect among three tested bacteria (p<0.05). Conclusions: The extract was labeled as an antibacterial agent against the representative of three foodborne bacteria, Invitro. The common effective concentrations of the extract (12.5, and 6.25%) is recommended for further research, as food additive, to remedy digestive ailments related to E. coli, S. typhimurium and Sh. dysenteriae

Keywords:

E. coli; Pathogen; Salmonella typhimurium; Shigella dysenteriae; Withania somnifera

References

[1] Cragg GM, Newman DJ, Snader KM. Natural products in drug discovery and development. J. Nat. Prod., 1997, 60: 52-60. DOI:https://doi.org/10.1021/np9604893

[2] Shahriari R, Salari S, Shahriari S. In vitro study of concentration-effect and time-course pattern of white alum on Escherichia coli O157:H7 growth. Afr. J. Tradit. Complement. Altern. Med., 2017, 14: 311-318. DOI:https://doi.org/10.21010/ajtcam.v14i2.32

[3] Ekor M. The growing use of herbal medicines: issues relating to adverse reactions, challenges in monitoring safety. Front. Pharmacol., 2013, 4: 177. DOI:https://doi.org/10.3389/fphar.2013.00177

[4] Afshar (Sistani) I. Sistan Traditional Medicine. Zabol, Zabol University Press, 2003: 45-63. (Text in Persian)

[5] Naseri nia MH, Nazerian NM, Naseri nia I. A survey on variety of common traditional treatments in Sistan and Baluchistan. J. Islam. and Iran. Trad. Med., 2013; 4: 55-66.

[6] Beigomi M, Ghods Rohani M, Mohammadifar MA, Hashemi M, Valizadeh M, Ghanati K. Comparison of textural and sensory characteristics of ultrafiltrated white cheese produced by paneer bad (Withania coagulans) protease and fungal rennet. Iranian Journal of Nutrition Sciences & Food Technology, 2013, 8: 253-262. (Text in Persian)

[7] Hemalatha S, Wahi AK, Singh PN, Chansouria JP. Hypolipidemic activity of aqueous extract of Withania coagulans Dunal in albino rats. Phytother. Res., 2006, 20: 614-617. DOI:https://doi.org/10.1002/ptr.1916

[8] Jaiswal D, Rai PK, Watal G. Antidiabetic effect of Withania coagulans in experimental rats. Indian J. Clin. Biochem., 2009, 24: 88-93. DOI:https://doi.org/10.1007/s12291-009-0015-0

[9] Halamova K, Kokoska L, Polesny Z, Macakova K, Flesar J, Rada V. Selective in vitro growth inhibitory effect of Withania somnifera on human pathogenic bacteria and bifido bacteria. Pak. J. Bot., 2013, 45: 667-670.

[10] Girish KS, Machiah KD, Ushanandini S, Harish Kumar K, Nagaraju S, Govindappa M. et al. Antimicrobial properties of a non-toxic glycoprotein (WSG) from Withania somnifera (Ashwagandha). J. Basic Microbiol., 2006, 46: 365-374. DOI:https://doi.org/ 10.1002/jobm.200510108

[11] Arora S, Dhillon S, Rani G, Nagpal A. The in vitro antibacterial/synergistic activities of Withania somnifera extracts. Fitoterapia, 2004, 75: 385-388. DOI:https://doi.org/10.1016/j.fitote.2004.01.002

[12] Quinn PJ, Markey BK, Carter ME, Donnelly WJC, Leonar FC, Hartigan P. et al. Veterinary Microbiology and Microbial Diseases. 2nd ed. USA:Wiley-Blackwell Publication, 2011: 263-287.

[13] Priyanka B, Patil RK, Dwarakanath S. A review on detection methods used for foodborne pathogens. Indian J. Med. Res., 2016, 144: 327-338. DOI:https://doi.org/10.4103/0971-5916.198677

[14] Chazarra S, Sidrach L, López-Molina D, Rodríguez-López JN. Characterization of the milk-clotting properties of extracts from artichoke (Cynara scolymus, L.) flowers. Int. Dairy J., 2007, 17: 1393-1400. DOI:https://doi.org/10.1016/j.idairyj.2007.04.010

[15] Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical. Biochem., 1976, 72: 248-254. DOI:https://doi.org/10.1006/abio.1976.9999

[16] Naroyi V, Salari S. Classification of resistance of Escherichia coli isolated from poultry with colibacillosis to the complement. Alexandria Journal of Veterinary Sciences, 2017, 54: 13-16. DOI:https://doi.org/10.5455/ajvs.264500

[17] Mishra LC, Singh BB, Dagenais S. Scientific basis for the therapeutic use of Withania somnifera (ashwagandha): a review. Altern. Med. Rev., 2000, 5: 334-346. PMID: 10956379

[18] Bussmann RW, Malca-Garcia G, Glenn A, Sharon D, Chait G, Diaz D. et al. Minimum inhibitory concentrations of medicinal plants used in Northern Peru as antibacterial remedies. J. Ethnopharmacol., 2010, 132: 101-108. DOI:https://doi.org/10.1016/j.jep.2010.07.048

[19] Kang CG, Hah DS, Kim CH, Kim YH, Kim E, Kim JS. Evaluation of antimicrobial activity of the methanol extracts from 8 traditional medicinal plants. Toxicol. Res., 2011, 27: 31-36. DOI:https://doi.org/10.5487/tr.2011.27.1.031

[20] Bokaeian M, Saeidi S. Evolution of antimicrobial activity of leaf extract of Withania somnifera against antibiotic resistanct Staphylococcus aureus. Zahedan J. Res. Med. Sci., 2015, 17: e1016.

[21] Owais M, Sharad KS, Shehbaz A, Saleemuddin M. Antibacterial efficacy of Withania somnifera (ashwagandha) an indigenous medicinal plant against experimental murine salmonellosis. Phytomedicine, 2005, 12: 229-235. DOI:https://doi.org/10.1016/j.phymed.2003.07.012

[22] Kumari M, Gupta RP. In vitro antibacterial effect of Withania somnifera root extract on Escherichia coli. Vet. World, 2015, 8: 57-60. DOI: 10.14202/vetworld.2015.57-60

[23] Anbalagan K, Sadique J. Role of prostaglandins in acute phase proteins in inflammation. Biochem. Med., 1984, 31: 236-245. PMID: 6202298

[24] Kamijo M, Kanazawa T, Funaki M, Nishizawa M, Yamagishi T. Effects of Rosa rugosa petals on intestinal bacteria. Biosci. Biotechnol. Biochem. 2008, 72: 773-777. DOI:https://doi.org/10.1271/bbb.70645

[25] Singariya P, Mourya KK, Kumar P. Antimicrobial activity of the crude extracts of Withania somnifera and Cenchrus setigerus In-vitro. Pharmacognosy Journal, 2012; 4: 60-65. DOI:https://doi.org/10.5530/pj.2012.27.10

[26] Sharma S, Dahanukar S, Karandikar S. Effects of long-term administration of the roots of ashwagandha and shatavari in rats. Indian drugs, 1985, 29: 133- 139.

[27] Fatima I, Hussain T, Rafay M, Akram M, Bano S, Shabbir S. Evaluation of antioxidant activity of leaves and fruits extracts of five medicinal plants. Pak. J. Pharm. Sci., 2017, 30: 1625-1628. PMID: 29084682

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