In Vitro Antibacterial Activity of Galla Chinensis Combined with Different Antibacterial Drugs against Carbapenem-Resistant E.Coli

Authors

  • Zhizhi Xie Department of Laboratory Medicine, The Third Affliated Hospital of Sun Yat-sen University
  • Changzhi Xu Department of Laboratory Medicine, The Third Affliated Hospital of Sun Yat-sen University
  • Yanhua Yi Department of Emergency, The Third Affliated Hospital of Sun Yat-sen University
  • Donglin Zhu Department of Laboratory Medicine, The Third Affliated Hospital of Sun Yat-sen University
  • Yun Xi Department of Laboratory Medicine, The Third Affliated Hospital of Sun Yat-sen University

DOI:

https://doi.org/10.30564/jams.v2i4.1229

Abstract

Objective: To evaluate the antibacterial effects of meropenem and levofloxacin respectively combined with Galla chinensis on carbapenem-resistant Escherichia coli in vitro. Methods： The protocol was designed with checkerboard method and the carbapenem-resistant E.coli was isolated in our hospital. The minimum inhibitory concentrations(MICs) of G. chinensis alone and combined with 2 antimicrobial agents against carbapenem-resistant E.coli were determined by broth dilution method and the fractional inhibitory concentration index (FICI) was calculated according to MICs results. Result： the combined use of G. chinensis and meropenem (or levofloxacin) significantly decreased both MIC50 and MIC90; After the combination of G. chinensis and meropenem, the synergistic effect was 86.7%, and the additive effect was 13.3%, no irrelevant and antagonistic effects. After combined use of G. chinensis and levofloxacin, the synergistic effect was 66.7%, and the additive effect was 33.3%. No irrelevant and antagonistic effects. Conclusion： Galla chinensis combined with meropenem or levofloxacin has synergistic and additive antibacterial effect, with certain combined antibacterial activity.

Keywords:

Galla chinensis; Carbapenem resistance; Escherichia coli; combination therapy

References

[1] Ghotaslou R,Sadeghi MR,Akhi MT,et al.Prevalence and Antimicrobial Susceptibility Patterns of ESBL, AmpC and Carbapenemase-producing Enterobactericeae Isolated from Hospitalized Patients in Azerbaijan, Iran[J]. Iran J Pharm Res,2018,17(Suppl):79-88.

[2] Manning S,Lautenbach E,Tolomeo P,et al. Risk factors for Infection with Escherichia coli in nursing home residents colonized with Fluoroquinolone-resistant E. coli[J]. Infect Control Hosp Epidemiol,2015,36(5):575-7.

[3] Woodward S. E. coli: a brief overview[J]. Br J Nurs,2015,24(3):158,60.

[4] Nordmann P,Naas T,Poirel L.Global spread of Carbapenemase-Producing Enterobacteriaceae[J]. Emerg Infect Dis,2011,17(10):1791-8.

[5] Chen Meiling, Wang Jian, Zjou Hongxia,el al.Inhibitory effect of Chinese herbal medicine on multiple antibiotic-resistant bacteria[J].Acta Chin Med Pharm,2012,40(2):10-13. (in Chinese)

[6] Li Kaixuan, Zhao Xiaojun, Wei Juan, et al. In vitro antibacterial effect of single-flavored traditional Chinese medicine on multidrug-resistant Acinetobacter baumannii [J]. Chinese Journal of Microecology,2018,30(4):392-397.

[7] Qiao Caiyun,Li Jianke.Research progress of gallnut and gallnut tannin[J].Food Industry Science and Technology,2011,32(7):458-462.

[8] Eliopoulos GM, Moellering RC, Jr. Antibiotic synergism and antimicrobial combinations in clinical infections[J]. Rev Infect Dis. 1982,4(2):282-93.

[9] Li Zhongxing, Wang Xiuhua, Zhang Mingming, et al. In vitro antibacterial activity of ethanol extract of Galla Chinensis against 140 strains of enterococci[J]. Chinese Journal of Information on Traditional Chinese Medicine,2004,11(3):201-203.

[10] Zhang Yali, Huang Xiaoxu, Cai Meiqin. Advances in Antioxidation and Relieving Physical Fatigue of Lycium Barbarum Polysaccharides[J]. Journal of Shanghai Jiaotong University (Medical Sciences), 2015, 35 (6): 911-915.

[11] Zhu Le,Wang Xue,Xu Lingshan et al. The effect of Yangqi Wuwei chewable tablets on relieving physical fatigue[J]. Journal of Yanbian University Medical Journal, 2015, 38 (2): 102-105.

[12] Zhang Haiyue, Sun Wuwen, Kang Yuanhuan et al. Inhibitory activity of Chinese herbal extracts on levofloxacin-resistant Escherichia coli[J]. Chinese Journal of Preventive Veterinary Medicine, 2016, 38 (11): 884-888.

[13] Wang Lingngjing, Zeng Jianming, Lu Yang et al. Effect of traditional Chinese medicine on multidrug-resistant Pseudomonas aeruginosa efflux pump[J]. Journal of molecular diagnosis and therape, 2017, 9 (6): 401-407.

[14] Tian Zhexian，Xuexian Yi ，Anna Cho , et al. CpxR activates MexAB-OprM efflux pump expression and enhances antibiotic resistance in both laboratory and clinical nalB-type isolates of pseudomonas aeruginosa[J].Plos Pathogens，2016，12（10）：e1005932.

[15] Zhang Guanghui, Cao Xueping, Yu Jing, et al. Epidemiology and drug resistance mechanism of carbapenem-resistant Acinetobacter baumannii clinical isolates[J]. Laboratory Medicine，2015，30（1）：53-57.

[16] Gao Yihan,Xie Xiaopeng,Xie Mingjie.Effects of ciprofloxacin combined with different traditional Chinese medicines on the mutation selection window of Staphylococcus aureus[J]. Chinese Journal of Biochemical Pharmaceutics，2016，36（5）：192-194.

[17] Tan Junqing, Li Yiwen, Wang Kangxi, et al. Antibacterial activity of cefoperazone-sulbactam combined with traditional Chinese medicine on pan-drug resistant Acinetobacter baumannii[J]. Laboratory Medicine，2016，31（5）：350-354.

Downloads

Issue

Vol. 2 , Iss. 4 (October 2019)

Article Type

Research Article

License

Copyright and Licensing

The authors shall retain the copyright of their work but allow the Publisher to publish, copy, distribute, and convey the work.

Journal of Advances in Medicine Science publishes accepted manuscripts under Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). Authors who submit their papers for publication by Journal of Advances in Medicine Science agree to have the CC BY-NC 4.0 license applied to their work, and that anyone is allowed to reuse the article or part of it free of charge for non-commercial use. As long as you follow the license terms and original source is properly cited, anyone may copy, redistribute the material in any medium or format, remix, transform, and build upon the material.

License Policy for Reuse of Third-Party Materials

If a manuscript submitted to the journal contains the materials which are held in copyright by a third-party, authors are responsible for obtaining permissions from the copyright holder to reuse or republish any previously published figures, illustrations, charts, tables, photographs, and text excerpts, etc. When submitting a manuscript, official written proof of permission must be provided and clearly stated in the cover letter.
The editorial office of the journal has the right to reject/retract articles that reuse third-party materials without permission.

Journal Policies on Data Sharing

We encourage authors to share articles published in our journal to other data platforms, but only if it is noted that it has been published in this journal.