Polysaccharide from Fruits of Physalis Alkekengi L. Enhances Antitumor Efficacy by a DNA Vaccine

Authors

  • Yun Xi Department of Laboratory Medicine, The Third Affiliated Hospital of Sun Yat-sen University
  • Donglin Zhu Department of Laboratory Medicine, The Third Affiliated Hospital of Sun Yat-sen University
  • Jing Huang Department of Laboratory Medicine, The Third Affiliated Hospital of Southern China Medical University
  • Jingping Liu Department of Laboratory Medicine, The Third Affiliated Hospital of Southern China Medical University
  • Hua Li Department of Laboratory Medicine, The Third Affiliated Hospital of Southern China Medical University
  • Gang Xiao Department of Laboratory Medicine, The Third Affiliated Hospital of Southern China Medical University

DOI:

https://doi.org/10.30564/jams.v1i4.291

Abstract

 Physalis. alkekengi fruit has long been used in traditional Chinese medicine for tumor therapy. In the present study, using plasmids that encode ovalbumin (OVA) gene, we investigate the adjuvant activity of a polysaccharide fraction (PPSB) isolated from P.alkekengi fruit. Formulation by simple procedures of mixing of the OVA-encoding pCI-neo-sOVA plasmid with PPSB not only induced specific antibody responses, but also induced antigen-specific cytotoxic T lymphocyte (CTL) responses (Graph abstract). Furthermore, immunization using this vaccine prevented the growth of OVA-expressing B16-OVA tumor cell growth in the immunized mice. Thus, we provide evidence supporting the adjuvant activity of PPSB in DNA vaccine against tumor.

Keywords:

Adjuvant; DNA vaccine; Physalis alkekengi L.; Polysaccharide

References

[1] A. Stachyra, A. Gora-Sochacka, A. Sirko. DNA vaccines against influenza[J]. Acta biochimica Polonica, 2014(61):515-522.

[2] J.R. Greenland, N.L. Letvin. Chemical adjuvants for plasmid DNA vaccines[J]. Vaccine, 2007(25):3731-3741.

[3] J. Zhou, & Wang, L. Analysis of nutrient composition in the fruit of Physalis alkekengi L[J]. Journal of Nutrition, 1997(19):243-245.

[4] H. Yang, S. Han, D. Zhao, G. Wang. Adjuvant effect of polysaccharide from fruits of Physalis alkekengi L. in DNA vaccine against systemic candidiasis[J]. Carbohydr Polym, 2014(109):77-84.

[5] C.P. Committee. Pharmacopoeia of The People’s Republic of China[M]. China Medical Science Press, Beijing,2010.

[6] H. Tong, Liang, Z., & Wang, G. Structural characterization and hypoglycemicactivity of a polysaccharide isolated from the fruit of Physalis alkekengi L[J]. Carbo-hydrate Polymers, 2008(71):316-323.

[7] G. Xiao, X. Li, A. Kumar, Z. Cui. Transcutaneous DNA immunization following waxing-based hair depilation elicits both humoral and cellular immune responses, European journal of pharmaceutics and biopharmaceutics[J].Official Journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 2012(82):212-217.

[8] R. Toll, U. Jacobi, H. Richter, J. Lademann, H. Schaefer, U. Blume-Peytavi, Penetration profile of microspheres in follicular targeting of terminal hair follicles[J]. The Journal of Investigative Dermatology, 2004(123):168-176.

[9] G. Chaouat. The Th1/Th2 paradigm: still important in pregnancy?[C]. Seminars in Immunopathology, 2007(29):95-113.

[10] J. Pol, N. Bloy, F. Obrist, A. Eggermont, J. Galon, W. Herve Fridman, I. Cremer, L. Zitvogel, G. Kroemer, L. Galluzzi. Trial Watch: DNA vaccines for cancer therapy[J]. Oncoimmunology, 2014(3):e28185.

[11] S.G. Reed, S. Bertholet, R.N. Coler, M. Friede. New horizons in adjuvants for vaccine development[J]. Trends in Immunology, 2009(30):23-32.

[12] S.G. Reed, M.T. Orr, C.B. Fox. Key roles of adjuvants in modern vaccines[J]. Nature Medicine, 2013(19):1597-1608.

[13] S.P. Smeekens, M.S. Gresnigt, K.L. Becker, S.C. Cheng, S.A. Netea, L. Jacobs, T. Jansen, F.L. van de Veerdonk, D.L. Williams, L.A. Joosten, C.A. Dinarello, M.G. Netea. An anti-inflammatory property of Candida albicans beta-glucan: Induction of high levels of interleukin-1 receptor antagonist via a Dectin-1/CR3 independent mechanism[J]. Cytokine, 2014(71):215-222.

[14] D. Gordon, P. Kelley, S. Heinzel, P. Cooper, N. Petrovsky. Immunogenicity and safety of Advax, a novel polysaccharide adjuvant based on delta inulin, when formulated with hepatitis B surface antigen: A randomized controlled Phase 1 study[J]. Vaccine, 2014(32):6469-6477.

[15] P.s.C.o.A.o.S.a. Technology, Report to the President on reengineering the influenza vaccine production enterprise to meet the challenges of pandemic influenza[EB/OL].http://www.whitehouse.gov/sites/default/files/microsites/ostp/PCAST-Influenza-Vaccinology-Report.pdf, 2010.

Downloads

Issue

Article Type

Research Article