Milk Fatty-Acid Profile after Feeding Increasing Doses of a Mixture of Soybean and Linseed Oils to Pasture Dairy Cows

Authors

  • Liliana Elisabet Antonacci Instituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Balcarce, Area de Producción Animal, Balcarce, Argentina
  • Gerardo Antonio Gagliostro Instituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Balcarce, Area de Producción Animal, Balcarce, Argentina

DOI:

https://doi.org/10.30564/jzr.v1i3.1986

Abstract

The goal was to determine the effect of growing intake of a mixture (75:25) of soybean (SoOi) and linseed (LiOi) oils on milk production and composition and milk fatty-acid (MF-A) profile in grazing dairy cows. Twenty-four Holstein cows were assigned to 4 treatments in a completely randomized design with three weeks of adaptation to oil doses and one week of experimental measurements. On a dry matter (DM) basis, cows were fed pasture (63%), energy concentrate (37%) and the SO-LO oil mixture at zero (Tr0%), 2% (Tr2%), 4% (Tr4%) and 6% (Tr6%) of total DM intake equivalent to 0, 0.36, 0.72 and 1.08 kg/cow/day of the oil mixture. The oil mixture was manually mixed-up to the concentrate (7.04 kg DM cow/day) and supplied by halves during each milking time without refusals. Pasture (P = 0.49) and total DM intakes (P = 0.31) were similar between treatments averaging 11.27 and 18.85 kg DM cow-1 day-1 respectively. Milk output (22.71 kg/cow/day) was not affected (P = 0.46). Milk fat content reduced linearly (P < 0.05) from 3.20 (Tr0%) to 2.67 g 100 g-1 (Tr6%) without effects (P = 0.73) on fat or fat corrected milk (4%FCM) yields. Milk protein concentration (P < 0.56) or yields (P < 0.11 were not affected. Lactose contents tended (P < 0.08) to be higher in oil supplemented cows and milk urea nitrogen was not affected (P = 0.14). The basal (Tr0%) concentration (g /100 g MF-A) of totaly hypercholesterolemic MF-A (C12:0, C14:0 and C16:0) of milk averaged 38.93 and decreased linearly (P < 0.0001) with oil intake to 37.81 (Tr2%), 31.59 (Tr4%) and 29.18 (Tr6%). Levels of elaidic (trans-9 C18:1) and trans-10 C18:1 MF-A resulted low-slung in the basal (Tr0%) milk (0.21 and 0.20 g/100g MF-A, respectively) but increased linearly (P < 0.0001) after oil intake reaching the maximum values at Tr6% (0.73 and 2.23 g/100g MF-A, respectively). Milk concentration (g/100g MF-A) of vaccenic acid (trans-11 C18:1, VA) averaged 3.63 in Tr0% and increased linearly (P < 0.0001) with oil intake reaching 4.97, 7.05 and 8.38 in Tr2%, Tr4% and Tr6%, respectively. Basal concentration of rumenic acid (cis-9. trans-11 C18:2, RA) was 2.28 g/100g MF-A and increased linearly (P < 0.0001) with increased oil dose resulting in maximal plateau in Tr4% (3.88) and Tr6% (3.89). The basal  atherogenic index (AI) of milk was 1.87 and linearly decreased (P < 0.01) to 1.64 (Tr2%), 1.18 (Tr4%) and 0.95 (T6%) after oil intake. The basal Ω6/Ω3 ratio (3.57) was no different (P > 0.05) from T2% (3.37) but was upper (P < 0.05) in Tr4% (4.41) and Tr6% (4.63) remaining under the recommended value of 5:1. Taken together the results suggest that feeding a blend (75:25; SoOi:LiOi) of SiOi an LiOi oils at 4% of total DM intake to pasture dairy cows maximize the milk RA content with a concomitant decrease in the hypercholesterolemic MF-A of milk maintaining a beneficial for health Ω6/Ω3 ratio with low levels of the detrimental trans-9 C18:1 and trans-10 C18:1.

Keywords:

Polyunsaturated oils; Vegetable oils; Rumenic acid

References

[1] Chilliard Y., Ferlay A., Mansbridge, R.M. and Doreau, M. (2000). Ruminant Milk Fat Plasticity: Nutritional Control of Saturated, Polyunsaturated, trans and Conjugated Fatty Acids. Annales de Zootechnie, 49, 181-205.

[2] Ulbright, T.L.V. and Southgate, D.A.T. (1991). Coronary Heart Disease: Seven Dietary Factors. Lancet, 338, 985-992.

[3] Tholstrup, T., Ehnholm, C., Jauhiainen, M., Petersen, M., Hoy, C.E., Lund, P. and Sandstrom, B. (2004). Effects of Medium-Chain Fatty Acids and Oleic Acid on Blood Lipids. Lipoproteins, Glucose, Insulin and Lipid Transfer Protein Activities. American Journal Clinical Nutrition, 79, 564-569.

[4] Parodi, P.W. (1999). Conjugated Linoleic Acid and Other Anticarcinogenic Agents of Bovine Milk Fat. Journal of Dairy Science, 82, 1339-1349.

[5] Gagliostro, G.A. (2004). Nutritional Control of Conjugated Linoleic Acid (CLA) Content in Milk and in Natural and Functional Foods. 2. Production of CLA-Enriched Milk in the Dairy Cow. Revista Argentina de Producción Animal, 24 (3-4), 137-163.

[6] Antonacci, L.E., Gagliostro, G.A., Cano, A.V. and Bernal, C.A. (2017). Effects of Feeding Combinations of Soybean and Linseed Oils on Productive Performance and Milk Fatty Acid Profile in Grazing Dairy Cows. Agricultural Sciences. 8. 984-1002.https://doi.org/10.4236/as.2017.89072

[7] Kritchevsky, D., Tepper, S.A., Wright, S., Tso., P. and Czarnecki, S.K. (2000). Influence of Conjugated Linoleic Acid (CLA) on Establishment and Progression of Atherosclerosis in Rabbits. Journal American College Nutrition, 19, 472S-477S.

[8] Valeille, K., Ferezou, J., Amsler, G., Quignard-Boulange, A., Parquet, M., Gripois, D., Dorovska-Taran, V. and Martin, J.C. (2005). A cis-9, trans-11-Conjugated Linoleic Acid-Rich Oil Reduces the Outcome of Atherogenic Process in Hyperlipidemic Hamster. American Journal of Physiology Heart and Circulating Physiology, 289, H652-659.

[9] Gagliostro, G.A. (2004). Nutritional Control of Conjugated Linoleic Acid (CLA) Content in Milk and in Natural and Functional Foods. 1. Effects on Human Health. Revista Argentina de Producción Animal, 24 (3-4), 113-136.

[10] Stachowska, E. (2008). Conjugated Dienes of Linoleic Acid and Tumorigenesis. Annales of Academic Medical Stetin, 54. 122-125.

[11] Shiraishi, R., Iwakiri, R. Fujise, T., Kuroki. T. Kakimoto, T., Takashima, T. Sakata, Y., Tsunada, S., Nakashima, Y., Yanagita, T. and Fujimoto, K. (2010). Conjugated Linoleic Acid Suppresses Colon Carcinogenesis in Azoxymethane-Pretreated Rats with Long-Term Feeding of Diet Containing Beef Tallow. Journal of Gastroenterology, 45, 625-635.

[12] Kim, J. H., Kim, Y. Kim, Y.J. and Park, Y. (2016). Conjugated Linoleic Acid: Potential Health Benefits as a Functional Food Ingredient. Annual Review of Food Science and Technology, 7, 221-244.

[13] Kelly, G.S. (2001). Conjugated Linoleic Acid: a Review. Alternative Medicine Review, 6, 367-382.

[14] Bhattacharya, A., Rahman, M.M., McCarter, R., O'Shea, M. and Fernandes, G. (2006). Conjugated Linoleic Acid and Chromium Lower Body Weight and Visceral Fat Mass in High-Fat-Diet-Fed Mice. Lipids, 41, 437-444.

[15] Stanton, C., Murphy, J., McGrath, E. and Devery, R. (2003). Animal Feeding Strategies for Conjugates Linoleic Acid Enrichment of Milk. In: Advances in Conjugated Linoleic Acid in Food. J.L Sébédio. W.W. Christie. R. Adloff (Eds.). AOCS Press. Champaign. Illinois. 2. PP 123-145.

[16] Gagliostro, G.A. Garciarena, D.A., Rodriguez, M.A. and Antonacci, L.E. (2017) Feeding Polyunsaturated Supplements to Grazing Dairy Cows Improve the Healthy Value of Milk Fatty Acids. Agricultural Sciences, 8. 759-782. https://doi.org/10.4236/as.2017.88057

[17] Schroeder, G.F., Gagliostro, G.A., Bargo, F., Delahoy, J.E. and Muller, L.D. (2004). Effects of Fat Supplementation on Milk Production and Composition by Dairy Cows on Pasture: a Review. Livestock Production Science, 86, 1-18.

[18] Fernandez, H.H. (1999). Estimation of Herbage Allowance. Course Principles of Nutrition and Supplementation in Grazing Cattle. INTA EEA Balcarce. June 1999.

[19] Meijs, J.A.C., Walters, R.J.K. and Keen, A. (1982). Sward Methods. In: Herbage Intake Handbook. British Grassland Society, 11-36.

[20] Horneck, A. D. and Miller, R. O. (1998). Determination of Total Nitrogen in Plant Tissue. In: Karla. Y. P. Ed: Handbook of Reference Methods of Plants Analysis CRC. Press. pp. 75-83.

[21] Morris, L.D. (1948). Quantitative Determination of Carbohydrates with Dreywood´s Anthrone Reagent. Science, 107, 254-255.

[22] Komarek, A.R., Robertson, J.B. and Van Soest, P.J. (1994). Comparison of the Filter Bag Technique to Conventional Filtration in the van Soest NDF Analysis of 21 Feeds. Proceeding National Conference on Forage Quality. Evaluation and Utilization. Fahey, G.C. Jr. (Ed). Nebraska University. Lincoln. NE. P 02.

[23] Komarek, A.R. (1993). An Improved Filtering Technique for the Analysis of Neutraldetergent Fiber and Acid Detergent Fiber Utilizing the Filter Bag Technique. Journal of Animal Science, 71, 824-829.

[24] AOAC. (2006). Official Methods of Analysis of the Association of Official Agricultural Chemists. 18th Edn. (1st revision). AOAC International, Gaithersburg. MD. USA.

[25] McRae, J.E. and Armstrong, D.G. (1968). Enzyme Method for Determination of Alpha-Linked Glucose Polymers in Biological Materials. Journal of Science and Food Agriculture, 19, 578-581.

[26] AOAC. (1999). Official Methods of Analysis of the Association of Official Agricultural Chemists. 18th Edn. (1st revision). AOAC International. Gaithersburg. MD. USA.

[27] Wolff, R. L. (1995). Content and Distribution of trans 18:1 Acids in Ruminant Milk and Meat Fats. Their Importance in European Diets and their Effect on Human Milk. ‎Journal of American Oil and Chemistry Society, 72, 259-272.

[28] Bannon, C. D., Breen, G. J., Craske, J. D., Hai. N. T., Harper, N. L. and O’Rourke, K. L. (1982). Analysis of Fatty Acid Methyl Esters with High Accuracy and Reliability. III. Literature Review of and Investigations into the Development of Rapid Procedures for the Methoxide Catalysed Methanol of Fats and Oils. Journal of Chromatography, Amsterdam, 247. n. 1. p. 71- 89.

[29] Masson, L. T., Alfaro, C., Camilo, A., Carvalho, P., Illesca, R., Torres, M., Tavares do Carmo, J., Mancini-Filho, A. and Bernal, C.A. (2015). Fatty Acid Composition of Soybean/Sunflower Mix oil, Fish Oil and Butterfat Applying the AOCS Ce 1j-07 Method with a Modified Temperature Program. Grasas y Aceites, 66, e064. ISSN-L: 0017-3495. doi: http://dx.doi.org/10.3989/gya.0692141

[30] SAS/STAT® User’s Guide. 2002-2010 by SAS Institute Inc. Cary. NC. USA.

[31] Minson, D.J. (1990). Forage in Ruminant Nutrition. Academic Press Inc., San Diego, California. 482 pp.

[32] Leaver, J.D. (1985). Herbage Intake Handbook. British Grassland Society. Hurley 1982. 143 pp.

[33] Verité, R. and Journet, M. (1970). Influence of Water Content and Dehydration of the Forage on Its Dietary Value for Dairy Cows. Annales de Zootechnie, 10, 269-277.

[34] Loor, J.J., Ferlay, A., Ollier, A., Doreau, M. and Chilliard, Y. (2005). Relationship Among trans and Conjugated Fatty Acids and Bovine Milk Fat Yield due to Dietary Concentrate and Lindseed Oil. Journal of Dairy Science, 88. 726-740.

[35] Flowers, G., Ibrahim, S.A. and AbuGhazaleh, A.A. (2008). Milk Fatty Acid Composition of Grazing Dairy Cows when Supplemented with Linseed oil. Journal of Dairy Science, 91,722–730.

[36] Bu, D.P., Wang, J.G., Dhiman, T.R. and Liu, S.J. (2007). Effectiveness of Oils Rich in Linoleic and Linolenic Acids to Enhance Conjugated Linoleic Acid in Milk from Dairy Cows. Journal of Dairy Science, 90, 998-1007.

[37] Glasser, F., Ferlay, A. and Chilliard, Y. (2008). Oilseed Lipid Supplements and Fatty Acid Composition of Cow Milk: a Meta-Analysis. Journal of Dairy Science, 91, 4687-4703.

[38] Martinez, M.G. (2010). Modulation of the Fatty Acid Composition of Bovine and Caprine Milk through Supplementation with Soybean and Fish Oil. Master Science Thesis, Faculty of Agrarian Sciences, National University of Mar de Plata, Argentina, 130 p.

[39]

[40] Martínez del Olmo, D. (2012). Suplementación de las Raciones para Vacas Lecheras de Alta Producción con Aceites de Origen Vegetal: Rendimientos Productivos y Reproductivos. Tesis doctoral. Universidad Complutense de Madrid. Facultad de Veterinaria. Departamento de Producción Animal. 122 p.p.

[41] Dewhurst, R.J., Shingfield. K.J., Lee. M.R.F. and Scollan, N.D. (2006). Increasing the Concentrations of Beneficial polyunsaturated Fatty Acids in Milk Produced by Dairy Cows in High-Forage Systems. Animal Feed Science and Technology, 131, 168-206. DOI: 10.1016/j.anifeedsci.2006.04.016.

[42] Jenkins, T.C. (1994). Feeding Fat to Dairy Cattle. In: Proceedings of the Dairy Herd Management Conference. University of Georgia. Athens. CA., 100-109

[43] Sutton, J.D., Knight, R., McAllan, A.B. and Smith, R.H. (1983). Digestion and Synthesis in the Rumen of Sheep Given Diets Supplemented with Free and Protected Oils. British Journal of Nutrition, 49, 419-432.

[44] Chilliard, Y., Ferlay, A. and Doreau, M. (2001). Effect of Different Types of Forages. Animal Fat or Marine Oils in Cow´s Diet on Milk Fat Secretion and Composition, Especially Conjugated Linoleic Acid (CLA) and Polyunsaturated Fatty Acids. Livestock Production Science, 70, 31-48.

[45] Rego, O.A., Rosa, H.J.D., Portugal, P., Cordeiro. R., Borba, A.E.S., Vouzela, C.M. and Bessa, R.J.B. (2005). The Effects of Supplementation with Sunflower and Soybeans Oils on the Fatty Acid Profile of Milk Fat from Grazing Dairy Cows. Animal Research, 54, 17-24.

[46] Chilliard, Y., Martin, C., Rouel. J. and Doreau, M. (2009). Milk Fatty acids in Dairy Cows fed Whole Crude Linseed, Extruded Linseed, or Linseed Oil , and their Relationship with Methane Output. Journal of Dairy Science, 92, 5199–5211.

[47] Pantoja, J., Firkins, J.L. and Eastridge, M.L. (1996). Fatty Acid Digestibility and Lactation performance by Dairy Cows Fed Fats Varying in Degree of Saturation. Journal of Dairy Science, 79, 429-437.

[48] Kim, E.J., Huws, S.A., Lee, M.R.F., Wood, J.D., Muetzel, S.M., Wallace, R.J. and Scollan. N.D. (2008). Fish Oil Increases the Duodenal Flow of Long Chain Polyunsaturated Fatty Acids and trans-11 18:1 and Decreases 18:0 in Steers via Changes in the Rumen Bacterial Community. Journal of Nutrition, 138, 889-896.

[49] Atkinson, R.L., Toone, C.D., Robinson, T.J., Harmon, D.L. and Ludden, P.A. (2009). Effects of Ruminal Protein Degradability and Frequency of Supplementation Nitrogen Retention, Apparent Digestibility and Nutrient Flux Across Visceral Tissues in Lambs Fed Low-Quality Forage. Journal of Animal Science, 10, 2246-2257.

[50] Dschaak, C. M., Noviandi, C. T., Eun, J.-S., Fellner, V., Young, A. J., ZoBell, D. R. and Israelsen, C. E. (2011). Ruminal Fermentation, Milk Fatty Acid Profiles, and Productive Performance of Holstein Dairy Cows fed 2 Different Safflower Seeds. Journal of Dairy Science, 94, 5138–5150.

[51] Doreau, M. and Chilliard, Y. (1997). Effects of Ruminal or Postruminal Fish Oil Supplementation on Intake and Digestion in Dairy Cows. Reproduction Nutrition Dévelopmènt, 37, 113–124.

[52] Ueda, K., Ferlay, A., Chabrot, J., Loor, J. J., Chilliard, Y. and Doreau. M. (2003). Effect of Linseed Oil Supplementation on Ruminal Digestion in Dairy Cows Fed Diets with Different Forage:Concentrate Ratios. Journal of Dairy Science, 86, 3999–4007.

[53] Palmquist, D. L. (1984). Use of Fats in Diets for Lactating Dairy Cow. Fats in Animal Nutrition. Butterworths. London. pp. 357-381.

[54] Morand-Fehr, P., Chilliard, Y. and Bas, P. (1986) Impact of Including Fats in the Ration on Yield and Composition of Ruminant Milk. Institut National de la Recherche Agronomique, 64, 59-72.

[55] Rego, O. A., Alves, S. P., Antunes. L.M. S., Rosa, H. J. D., Alfaia C. F. M., Prates, J. A. M., Cabrita, A. R. J., Fonseca, A. J. M. and Bessa, R. J. B. (2009). Rumen Biohydrogenation-Derived Fatty Acids in Milk Fat from Grazing Dairy Cows Supplemented with Rapeseed, Sunflower, or Linseed Oils. Journal of Dairy Science, 92, 4530–4540.

[56] Dhiman, T.R., Satter, L.D., Pariza, M.W., Galli, M.P., Albright, K. and Tolosa, M.X. (2000). Conjugated Linoleic Acid (CLA) Content of Milk from Cows Offered Diets Rich in Linoleic and Linolenic Acid. Journal of Dairy Science, 83, 1016–1027.

[57] Alzahal, O., Odongo, N.E., Mutsvanqwa, T., Or-Rashid, M.M., Duffield, T.F., Baqq, R., Dick. P, Vessie. G. and McBride, B.W. (2008). Effects of Monensin and Dietary Soybean Oil on Milk Fat Percentage and Milk Fatty Acid Profile in Lactating Dairy Cows. Journal of Dairy Science, 91 (3). 1166-1174.

[58] Huang, Y., Schoonmaker, J.P., Bradford, B.J. and Beitz, D.C. (2008). Response of Milk Fatty Acid Composition to Dietary Supplementation of Soy Oil, Conjugated Linoleic Acid, or Both. Journal of Dairy Science, 91, 260–270.

[59] Piperova, L.L., Teter, B.B., Bruckental, I., Sampugna, J., Mills, S.E., Yurawecz., M.P., Fritsche, J. Ju, K and Erdman, R.A. (2000). Mammary Lipogenic Enzyme Activity, trans Fatty Acids and Conjugated FattyAacids are Altered in Lactating Dairy Cows fed a Milk-Fat Depressing Diet. Journal of Nutrition, 130, 2568-2574.

[60] Whitlock, L.A., Schingoethe, D.J., AbuGhazaleh, A.A., Hippen, A.R. and Kalscheur, K.R. (2006). Milk Production and Composition from Cows Fed Small Amounts of Fish Oil and Extruded Soybean. Journal of Dairy Science, 89, 3972-3980.

[61] Shingfield, K. J., Reynolds, C. K., Hervas, G., Griinari, J. M., Grandison, A. S. and Beever, D.E. (2006). Examination of the Persistency of Milk Fatty Acid Composition Responses to Fish Oil and Sunflower Oil in the Diet of Dairy Cows. Journal of Dairy Science, 89, 714–732.

[62] Chilliard. Y., Ferlay, A,. Loor, J., Rouel. J. and Martin, B. (2002). Trans and Conjugated Fatty Acids in Milk from Cows and Goats Consuming Pasture or Receiving Vegetable Oils or Seeds. Italian Journal of Animal Science, 1, 243-254.

[63] Gagliostro, G.A. and Chillard, Y. (1992). Protected Lipid Utilization in Dairy Cows Nutrition. I. Effects on Milk Production and Composition, Dry Matter and Energy Intake (A Review). Revista Argentina de Producción Animal, 12, (1). 1-15.

[64] Bargo, F., Muller, L.D., Kolver, E.S. and Delahoy, J.E. (2003). Invited Review: Production and Digestion of Supplemented Dairy Cows on Pasture. Journal of Dairy Science, 86, 1-42.

[65] Wu, Z. and Huber, J.T. (1994). Relationship Between Dietary Fat Supplementation and Milk Protein Concentration in Lactating Cows: A Review. Livestock Production Science, 39, 141-155.

[66] Storry, J.E. (1981). The Effect of Dietary Fat on Milk Composition. In: Recent Advances in Animal Nutrition. W. Haresing. (Eds). Butterworths. London. 3-33.

[67] Dunkley, W.L., Smith, N.E. and Franke, A.A. (1977). Effect of Feeding Protected Tallow on Composition of Milk and Milk fat. Journal of Dairy Science, 60, 1863-1869.

[68] Palmquist, D.L., Beaulieu, A.D. and Barbano, D.M. (1993). Feed and Animal Factors Influencing Milk Fat Composition. Journal of Dairy Science, 76, 1753–1771.

[69] Ward, A. T., Wittenberg, K. M. and Przybylski, R. (2002). Bovine Milk Fatty Acid Profiles Produced by Feeding Diets Containing Solin, Flax and Canola. Journal of Dairy Science, 85, 1191–1196.

[70] Garnsworthy, P.C. (1997). Fats in Dairy Cow Diets. In: Recent Advances in Animal Nutrition. pp 87–13. Cole. D.J.A. (eds.). Nottingham UniversityPress. Nottingham. UK.

[71] Vicente, G.R., Shelford, J.A., Peterson, R.G. and Khrishnamurti, C.R. (1984). Effects of Feeding Canola-Meal-Protected Tallow or Soy-Bean-Meal-Protected Tallow in the Low-Roughage Diet of Dairy Cows in Early Lactation. Canadian Journal of Animal Science, 64, 81.

[72] Gagliostro, G.A., Chilliard, Y. and Davicco, M.J. (1991). Duodenal Rapeseed Oil Infusion in Early and Mid-Lactation Cows. 3. Plasma Hormones and Mammary Apparent Uptake of Metabolites. Journal of Dairy Science, 74, 1893-1903.

[73] Chilliard, Y., Gagliostro, G.A., Fléchet. J., Lefaivre, J. and Sebastian, I. (1991). Duodenal Rapeseed Oil Infusion in Early and Mid Lactation Cows. 5. Milk Fatty Acids and Adipose Tissue Lipogenic Activities. Journal of Dairy Science, 74, 1844-1854.

[74] Christie, W.W. (1981). The Effects of Diet and Other Factors on the Lipid Composition of Ruminant Tissues and Milk. In: Lipid metabolism of ruminant animals. Christie W.W. (Ed). Pergamon Press. Oxford. 193-226.

[75] Mandebvu, P., Ballard, C.S., Sniffen, C.J., Carter, M.P., Wolford, H.M., Sato, T., Yabuuchi, Y., Bolck, E. and Palmquist, D.L. (2003). Effect of Feeding Calcium Salts of Long-Chain Fatty Acids from Palm Fatty Acid distillate or Soybean Oil to High Producing Dairy Cows on Milk Yield and Composition and on Selected Blood and Reproductive Parameters. Animal Feed Science and Technology, 108. 25-41.

[76] Ambrose. D. J., Kastelic, J. P., Corbett, R., Pitney, P.A., Petit, H.V., Small, J.A. and Zlkovic, P. (2006). Lower Pregnancy Losses in Lactating Dairy Cows Fed a Diet Enriched in Linoleic Acid. Journal of Dairy Science, 89, 3066–3074.

[77] Castañeda-Gutierrez, E., Benefield, B.C., de Veth, M.J., Santos, N.R.. Gilbert, R.O. Butler, W.R. and Bauman, D.E. (2007). Evaluation of the Mechanism of Action of Conjugated Linoleic Acid Isomers on Reproduction in Dairy Cows. Journal of Dairy Science, 90. 4253- 4264.

[78] Fuentes, M.C., Calsamiglia, S.S., Sánchez, C., González, A.. Newbold, J., Santos, J. E.P., Rodríguez-Alcalá, L.M. and Fontecha, J. (2008). Effect of Extruded Linseed on Productive and Reproductive Performance of Lactating Dairy Cows. Livestock Production Science, 113, 144-154.

[79] Chilliard, Y. and Ferlay, A. (2004). Dietary Lipids and Forages Interactions on Cow and Goat Milk Fatty Acid Composition and Sensory Properties. Reproduction Nutrition Dévelopmènt, 44. 467–492.

[80] Antonacci, L., Rodríguez, A., Castelli, L., Zampatti, M., Castañeda, R., Ceaglio, J. and Gagliostro, G.A. (2013). Suplementación con Mezcla de Aceites Vegetales y el Perfil en Acidos Grasos de Leche Bovina. Revista Argentina de Producción Animal. Vol. 33. Supl. 1.

[81] Casper, D.P., Shingoethe, D.J., Middaugh, R.P. and Baer, R.J. (1988). Lactational Responses of Dairy Cows to Diets Containing Regular and High Oleic Sunflower Seeds. Journal of Dairy Science, 71, 1267– 1274.

[82] Bauman, D.E. and Griinari, J.M. (2001). Regulation and Nutritional Manipulation of Milk Fat: Low-Fat Milk Syndrome. Livestock Production Science, 70, 15–29.

[83] Kelly, M.L., Berry, J.R., Dwyer, D.A., Griinari, J.M., Chouinard, P.Y., Van Amburgh, M.E. and Bauman, D.E. (1998). Dietary Fatty Acid Sources Affect Conjugated Linoleic Acid (CLA) Concentrations in Milk from Lactating Dairy Cows. Journal of Nutrition, 128, 881–885.

[84] Roy, A., Ferlay, A. and Chilliard, Y. (2007). Production of Butter Fat Rich in trans-10C18:1 for Use in Biomedical Studies in Rodents. Reproduction Nutrition Dévelopmènt, 46, 211-218.

[85] Hartoof, C.G., Noble, R.C. and Moore, J. H. (1973). Factors Influencing the Extent of Biohydrogenation of Linoleic Acid by Rumen Micro-Organisms in vitro. Journal of Science and Food Agriculture, 24. 961–970.

[86] Agazzi, A., Bayourthe, C., Nicot, M.C., Troegeler-Meynadier, A., Moncoulon, R. and Enjhalnbert, M. (2004). In Situ Ruminal Biohydrogenation of Fatty Acids from Extruded Soybeans: Effects of Dietary Adaptation and of Mixing with Lecithin or Wheat Straw. Animal Feed Science and Technology, 117, 165-175.

[87] Cruz-Hernandez, C., Kramer, J.K.G., Kennelly, J.J., Glimm, D.R., Sorensen, B.M., Okine, E.K., Goonewardene, L. A. and Weselake, R. J. (2007). Evaluating the Conjugated Linoleic Acid and Trans 18:1 Isomers in Milk Fat of Dairy Cows Fed Increasing Amounts of Sunflower Oil and a Constant Level of Fish Oil. Journal of Dairy Science, 90, 3786–3801.

[88] Hurtaud, C., Faucon, F., Couvreur, S and Peyraud, J.L. (2010). Linear Relationship Between Increasing Amounts of Extruded Linseed in Dairy Cow Diet and Milk Fatty Acid Composition and Butter Properties. Journal of Dairy Science, 93. 1429–1443.

[89] Mozaffarian, D., Katan, M. B., Ascherio, A., Stampfer, M. J., Willett, W.C. and Food, T. (2006). Trans Fatty Acids and Cardiovascular Disease. New England Journal of Medicine, 1601-1613.

[90] Dorfman, S.E., Laurent, D., Gounarides, J.S., Li, X., Mullarkey, T.L., Rocheford, E.C., Sari-Sarraf, F. (2009). Metabolic Implications of Dietary trans-fatty acids. Obesity, 17, 1200-1207.

[91] Chilliard, Y., Gasser, G., Enjalber,. F., Ferlay, A., Bocquier, F. and Schimidely, P.H. (2007). Conferencia: Resultados Recientes Sobre los Efectos de la Alimentación en la Composición en Ácidos Grasos de la Leche de Vaca. Cabra y Oveja. Revista Argentina de Producción Animal, 27(3). 197-213.

[92] Awad, A.B., Hermann, T., Fink, C.S., Horvath, P.J. (1995). 18:1 n7 Fatty Acids Inhibit Growth and Decreased Inositol Phosphate Release in HT-29 Cells Compared to n-9 Fatty Acids. Cancer Letters, 91, 55-61.

[93] Turpeinen, A.M., Mutanen, M., Aro, A., Salminen, I., Basu, S., Palmquist, D.L. and Griinari, J.M. (2002). Bioconversion of Vaccenic Acid to Conjugated Linoleic Acid in Humans. American Journal of Clinical Nutrition, 76, 504-510.

[94] Parodi, P.W. (2003). Conjugated Linoleic Acid in Food. In: Advances in Conjugated Linoleic Acid in Food. Volume 2. J.L Sébédio. W.W. Christie. R. Adloff (Eds.). AOCS Press. Champaign. Illinois. pp 101-122.

[95] Griinari, J.M. and Bauman, D.E. (1999). Biosynthesis of Conjugated Linoleic Acid and its Incorporation Into Meat and Milk in Ruminants. In: M. P. Yurawecz. M. M. Mossoba. J. K. G. Kramer. M. W. Pariza and G. J. Nelson (Eds.) Advances in Conjugated Linoleic Acid Research. pp 180-200. AOCS Press. Champaign.

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