Prevalence of Kudoa in Fish Fillets Caught in Para State

Authors

  • Marianna Vaz RODRIGUES UNESP – São Paulo State University (Unesp), Biotechnology , Alameda das Tecomarias s/n, 18607-440, Botucatu, São Paulo, Brazil
  • Patrícia Tidori MIURA Food Technology Laboratory, Food Science Department, University of Campinas, Cidade Universitária “Zeferino Vaz” s/n, 13083-862, Campinas, São Paulo, Brazil
  • Jéssica Fernandes de OLIVEIRA UNESP – São Paulo State University (Unesp), Biotechnology , Alameda das Tecomarias s/n, 18607-440, Botucatu, São Paulo, Brazil
  • Maria das Dores Correia Palha Universidade Federal Rural da Amazônia, Avenida Presidente Tancredo Neves 2501, 66077-830, Belém, Pará, Brazil
  • João Pessoa Araújo JÚNIOR UNESP – São Paulo State University (Unesp), Biotechnology , Alameda das Tecomarias s/n, 18607-440, Botucatu, São Paulo, Brazil

DOI:

https://doi.org/10.30564/jzr.v2i3.2092

Abstract

Kudoa is a myxozoan that causes myoliquefaction in marine fishes. Most of species only affect fish, but a K. septempunctata outbreak was reported in 358 people. Although many species of Kudoa are known, none was described in Brachyplatystoma filamentosum, Brachyplatystoma rousseauxii, Mugil curema, Plagioscion squamosissimus or Oxydoras niger until now. Due to the economic cost of eliminating seafood presenting myxozoan lesions, this study aimed to describe lesions found at necropsy and histopathology, as well as to detect this myxozoan by molecular techniques. For this purpose, were sampled 85 fish of the following species: Brachyplatystoma filamentosum, Brachyplatystoma rousseauxii, Mugil curema, Plagioscion squamosissimus, and Oxydoras niger from Colares and Vigia, Pará, Brazil. Necropsies were carried out to describe lesions and molecular techniques (PCR and sequencing) were applied for identification. Although muscle lesions were not observed at necropsy, histopathology revealed bacterial colonies, coagulative necrosis, dystrophic calcification, eosinophils, hemorrhage, parasitic cysts, protozoan, and vacuolization. After sequencing, K. shiomitsui (GENBANK: LC128646) was identified as the causative agent of fishes infection. Also, high parasitism of this myxozoan was observed in fishes sampled, i.e., 90 % in Colares and 100% in Vigia.

Keywords:

Myxozoan; Parasitism; Seafood; Sanitary inspection; Myoliquefaction

References

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Vol. 2 , Iss. 3 (July 2020)

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