Sex Hormones Changes in Blood and Their Effect on Fecundity of African Catfish (Clarias gariepinus Burchell, 1822) after Being Injected with Different Doses of Human Chorionic Gonadotropin (HCG) Hormone

Authors

  • Hamed H.E. Saleh Aquaculture Division, National Institute of Oceanography and Fisheries (NIOF), El-Fayoum, Egypt
  • Ahmed I. Semaida Animal Production Department, Faculty of Agriculture, Fayoum University, Egypt
  • Sayed R.S. Zidan Animal Production Department, Faculty of Agriculture, Fayoum University, Egypt
  • Ramadan M. Abou-Zied Animal Production Department, Faculty of Agriculture, Fayoum University, Egypt
  • Sobhy M. Allam Animal Production Department, Faculty of Agriculture, Fayoum University, Egypt

DOI:

https://doi.org/10.30564/jzr.v2i4.2189

Abstract

This study was conducted to investigate the effect of different dosesinjection of human chorionic gonadotropin (HCG) hormone on fecundityand serum sex hormones (FSH, LH, estrogen (E2), progesterone (P4),testosterone (T)) of African catfish (Clarias gariepinus). African catfishspawners were intermuscularly injected with different doses of HCG(500, 1500, 3000, 6000 IU/kg female), and group is not injected as acontrol; males were injected at half the female dose. The results showedthat, fish group injected by 6000 IU HCG/ kg female had the highestgonadsomatic index, absolute fecundity and relative fecundity, while,the lowest value of absolute fecundity and relative fecundity wererecorded with 500 IU HCG/ kg female. The group injected with thehighest amount of HCG (6000 IU/ kg female) recorded the lowest valuefrom egg diameter, while the highest egg diameter was observed in 500IU HCG/ kg female. In females, the group injected with 6000 IU HCG/kg female reflected the lowest level of FSH and the highest level of LHand the highest level of P4 compared to other treatments. Level of Trecorded the highest level with 1500 IU HCG/ kg female. The controlgroup reflected the highest level of FSH and E2, while the control groupreflected the lowest level of T and P4 level. In males, serum FSH, LH,P4 and E2 in male groups injected with HCG were relatively higher thanthose recorded in the control group. The highest level of T was recordedin treatment injected with the highest dose of HCG and decreased inother treatments until recorded the lowest level of T in the controlgroup. It was observed, HCG hormone has successfully and accelerateinduced spawning in African catfish (Clarias gariepinus) and increasedin reproductive performance with the increase in HCG dosage and ascompared to group not injected.

Keywords:

African catfish; HCG hormone; Gonadsomatic index; Fecundity; FSH; LH; Estrogen; Progesterone and testosterone

References

[1] Rottmann, R.W., Shireman, J.V., Chapman, F.A. Introduction to hormone-induced spawning of fish. SRAC Publication, 1991: 421.

[2] Zohar, Y., Munoz-Cueto, J.A.; Elizur, A., Kah,

[3] O.Neuroendocrinology of reproduction in teleost fish. Gen. Comp. Endocrinol., 2010, 165: 438-455.

[4] Planas, J.V., Swanson, P. Physiological function of gonadotropins in fish. In fish reproduction (eds. M.J. Rocha, A. Arukwe and B.G. Kapoor). Enfield, NH: Science Publishers, 2008: 37-66.

[5] Levavi-Sivan, B., Bogerd, J., Mananos, E.L., Gomez, A., Lareyre, J.J. Perspectives on fish gonadotropins and their receptors. Gen. Comp. Endocrinol., 2010, 165: 412-437.

[6] Yaron, Z. Endocrine control of gametogenesis and spawning induction in the carp. Aquaculture, 1995, 129(1-4): 49-73.

[7] Devlin, R.H., Nagahama, Y. Sex determination and sex differentiation in fish: an overview of genetic, physiological, and environmental influences. Aquaculture, 2002, 208(3-4): 191-364.

[8] Nagahama, Y. Endocrine regulation of gametogenesis in fish, Int. J. Develop. Biol., 1994, 38: 217-229.

[9] Borg, B. Androgens in teleost fishes, Comp. Biochem. Physiol., 1994, 109(C): 219-245.

[10] Patino, R., Yoshizaki, G., Thomas, P., Kagawa, H. Gonadotropic control of ovarian follicle maturation: the two-stage concept and its mechanisms, Comp. Biochem. Physiol., 2001, 129(B): 427-439.

[11] Olaleya, V.F. Review of reproduction and gamete management in the African catfish Clarias gariepinus(BURCHELL). Ife Journa/ of Science, 2005, 7(1): 63-70.

[12] Patino, R., Thomas, P.X. Effects of gonadotropin on ovarian intrafollicular processes during the development of oocyte maturational competence in a teleost, the Atlantic croaker: evidence for two distinct stages of gonadotropin control of final oocyte maturation. Biol. Reprod., 1990, 43(5): 818-27.

[13] York, W.S., Patino, R., Thomas, P. Ultrastructural changes in follicle cell-oocyte associations during development and maturation of the ovarian follicle in Atlantic croaker. Gen. Comp. Endocrinol., 1993, 92(3): 402-18.

[14] Srivastava, R.K., Van Der Kraak, G. Effects of activators of different intracellular signaling pathways on steroid production by goldfish vitellogenic ovarian follicles. Gen. Comp. Endocrinol., 1994, 93(2): 181-91.

[15] Ahmed, A.M.M. Effect of induced breeding of Clarias lazera using hormones of HCG. American Journal of Marine Research and Reviews, 2018, 1:2. DOI: 10.28933/ajmrr-2018-02-2801

[16] Doha, S., Hyne, A.M. Fecundity of Padma river hilsa, Hilsa ilisha Trans. Amer. Fish. Soc., 1970, 97(4): 350-355.

[17] Shafi, M., Quddus, M.M.A., Hossain, M. Studies on length weight relationship sex ratio, size composition, gears and abundance of Hilsa ilishain the river Padma, Dacca Univ. Stud. B., 1978, 26: 123-127.

[18] Dewan, S., Doha, S. Spawning and fecundity of certain pond fishes. Bangladesh J. Agril., 1979, 4(1): 1-8.

[19] Das, M., Dewan, S., Debnath, S.C. Studies on fecundity of Heteropneustes fossilis (Bloch) in a minipond of Bangladesh Agricultural University, Mymensingh. Bangladesh J. Agril. Sci., 1989, 16(1): 1-6.

[20] Nikolosky, G.V. The Ecology of fishes. Academic press, London and New York, 1963.

[21] Tseng, W.Y., Chan, K.L. The productive biology of the rabbit fish in Hong Kong. J. World Maricul. Soc., 1982, 13: 313-321.

[22] Mehrim, A.I., Abdelhamid, A.M., Radwan, I.A., Abdelhamid, A.F. Comparative study for different sources of reproductive stimulating materials and their effects on the reproductive performance of African catfish Clarias gariepinus (Burchell, 1822).

[23] Asian Journal of Animal and Veterinary Advances, 2014, 9(7): 414-427.

[24] SPSS. Statistical Package for Social Science (for Windows). Release 23 Copyright (C), SPSS Inc., Chicago, USA, 2015.

[25] Rottmann, R.W., Shireman, J.V., Chapman, F.A. Hormonal control of reproduction in fish for induced spawning. SRAC Publication No. 424, 1991.

[26] S h a r a f , S . M . E f f e c t s o f g o n a d o t r o p i n

[27] r e l e a s i n g h o r m o n e ( G n R H s ) a n d h u m a n

[28] chorionicgonadotropin (HCG) on ovarian development andplasma levels of sex steroids in African catfish (Clarias gariepinus). Egypt. J. of Appl. Sci., 2005, 20(7): 23-37

[29] Saadony, S.A.M.. Eldanasoury, M.A.. Ali, B.A.. Sharaf, S.M. Seasonal reproductive biology and artificial propagation of female African catfish (Clarias gariepinus) after hormonal stimulation.

[30] Journal of Animal, Poultry & Fish Production, Suez Canal University, 2014, (2): 21-31.

[31] Denusta, P.J.T., de Jesus-Ayson, E.G.T., Laron, M.A., Garcia, L.M.B. Effects of human chorionic gonadotropin (HCG) and handling stress on spermiation of silver perch Leiopotherapon plumbeus

[32] (Kner, 1864). J. Appl. Ichthyol., 2014, 30: 448-453.

[33] El-Hawarry, W.N., AbdEl-Rahman, S.H., Shourbela, R.M. Breeding response and larval quality of African catfish (Clarias gariepinus, Burchell 1822) using different hormones/hormonal analogues withdopamine antagonist. Egyptian Journal of Aquatic

[34] Research, 2016, 42: 231-239.

[35] Babin, P.J., Cerda, J., Lubzens, E. The fish oocyte: From Basic Studies to Biotechnological Applications. Springer, 2007.

[36] Bichi, A.H., Isyaku, S., Danba, E.P., Kurawa, I.A., Nayawo, A.A. Effect of brood stock size on egg fertilization, hatchability and survival rate of African catfish (Clarias gariepinus). Bayero Journal of Pure and Applied Sciences, 2014, 7(2): 150-154.

[37] Biran, J., Levavi-Sivan, B. Endocrine control of reproduction, Fish, Encyclopedia of Reproduction, 2nd edition. Elsevier Inc., 2018, 6: 362-367.

[38] Lubzens, E., Bobe, J.; Young, G., Sullivan, C. Maternal investment in fish oocytes and eggs: The molecular cargo and its contributions to fertility and early development. Aquaculture, 2017, 472: 107-143.

[39] Mylonas, C.C., Fostier, A., Zanuy, S. Broodstock management and hormonal manipulations of fish reproduction. General and Comparative Endocrinology, 2010, 165: 516-534.

[40] Sharaf, S.M. Effect of GnRHa, Pimozide and Ovaprim on ovulation and plasma sex steroid hormones in African catfish Clarias gariepinus. Theriogenology, 2012, 77(8): 1709-1716.

[41] Shourbela, R.M., Abd-El-latif, A.M., Abd-El-Azem, M.A. Induced spawning of African catfish, using (GnRHa) combined with Dopamine Antagonists. Benha Veterinary Medical Journal, 2014, 27(1): 25-35.

[42] Reading, B.C., Sullivan, V.C. Vitellogenesis in fishes. North Carolina (US): Elsevier Inc. Hlm, 2017: 1-12.

[43] Kim, D., Bae, J., Kim, E. Changes in sex steroid hormones and ovarian development during artificial maturation of female Eel, Anguilla japonica. Integrative Biosciences, 2007, 11: 117-124.

[44] Achionye-Nzeh, C.G., Obaroh, I. Ovaprim doses effects on eggs of African mudfish Clarias gariepinus. International Journal of Life Science and Pharma Research, 2012, 2: 6-9.

[45] Shokr, E.A.M. Effect of gonadotropin releasing hormone injection on physiological changes and reproductive hormones in Clarias gariepinus. Egyptian Journal of Aquatic Biology & Fisheries,

[46] , 24(1): 119-129.

[47] Mousavi1, S.E., Yousefian, M. Effects of exogenous

[48] hormones on plasma cortisol, sex steroid hormone and glucose levels in male and female grass carp, Ctenopharyngodon idellus, during the spawning induction. African Journal of Biotechnology, 2012, 11(36): 8920-8927.

[49] Miura, T., Miura, C.I. Molecular control mechanisms

[50] of fish spermatogenesis. Fish Physiol. Biochem., 2003, 28: 181-186.

[51] Mateos, J., Mananos, E., Martinez-Rodriguez, G., Carrillo, M., Querat, B., Zanuy, S. Molecular characterization of sea bass gonadotropin subunits (a, FSHb, and LHb) and their expression during the reproductive cycle. Gen. Comp. Endocrinol., 2003,

[52] : 216-232.

[53] Cacot, P., Eeckhoutte, P., Muon, D.T., Trieu, N.V., Legendre, M., Mariojouls, C., Lazard, J. Induced spermiation and milt management in Pangasius bocourti (Sauvage, 1880). Aquaculture, 2003, 215: 67-77.

[54] Schiavone, R., Zilli, L., Vilella, S., Fauvel, C. Human chorionic gonadotropin induces spermatogenesis and spermiation in 1-year-old European sea bass (Dicentrarchus labrax): assessment of sperm quality. Aquaculture, 2006, 255: 522-531.

[55] Asturiano, J.F., Perez, L., Garzon, D.L., Penaranda, D.S., Marco-Jimenez, F., Martinez-Llorens, S., Tomas, A., Jover, M. Effects of different methods for the induction of spermiation on semen quality in European eel. Aquac. Res., 2005, 36: 1480-1487.

[56] Asturiano, J.F., Marco-Jimenez, F., Perez, L., Balasch, S., Garzon, D.L., Penaranda, D. S., Vicente, J.S., Viudes-de-Castro, M.P., Jover, M. Effects of HCG as spermiation inducer on European eel semen quality. Theriogenology, 2006, 66: 1012-1020.

[57] Gallego, V., Mazzeo, I., Vilchez, M.C., Penaranda, D.S., Carneiro, P.C.F., Perez, L., Asturiano, J.F. Study of the effects of thermal regime and alternative hormonal treatments on the reproductive performance of European eel males (Anguilla anguilla) during induced sexual maturation. Aquaculture, 2012, 354-

[58] : 7-16.

[59] Tanaka, H., Kagawa, H., Ohta, H., Unuma, T., Nomura, K. The first production of glass eel in captivity: fish reproductive physiology facilitates great progress in aquaculture. Fish Physiol.

[60] Biochem., 2003, 28: 493-497.

[61] Kagawa, H., Tanaka, H., Ohta, H., Unuma, T., Nomura, K. The first success of glass eel production in the world: basic biology on fish reproduction advances new applied technology in aquaculture.

[62] Fish Physiol. Biochem., 2005, 31: 193-199.

[63] Zohar, Y., Mylonas, C.C. Endocrine manipulations of spawning in cultured fish: from hormones to genes. Aquaculture, 2001, 197: 99-136

[64] Chen, R.J., Huang, S.C., Chow, S.N., Hsieh, C.Y. Human chorionic gonadotropin pattern in maternal circulation. Amniotic fluid and fetal circulation in late pregnancy. J. Reprod. Med., 1993, 38: 151-154.

Downloads

Issue

Vol. 2 , Iss. 4 (October 2020)

Article Type

Articles

License

Copyright and Licensing

The authors shall retain the copyright of their work but allow the Publisher to publish, copy, distribute, and convey the work.

Journal of Zoological Research publishes accepted manuscripts under Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). Authors who submit their papers for publication by Journal of Zoological Research agree to have the CC BY-NC 4.0 license applied to their work, and that anyone is allowed to reuse the article or part of it free of charge for non-commercial use. As long as you follow the license terms and original source is properly cited, anyone may copy, redistribute the material in any medium or format, remix, transform, and build upon the material.

License Policy for Reuse of Third-Party Materials

If a manuscript submitted to the journal contains the materials which are held in copyright by a third-party, authors are responsible for obtaining permissions from the copyright holder to reuse or republish any previously published figures, illustrations, charts, tables, photographs, and text excerpts, etc. When submitting a manuscript, official written proof of permission must be provided and clearly stated in the cover letter.
The editorial office of the journal has the right to reject/retract articles that reuse third-party materials without permission.

Journal Policies on Data Sharing

We encourage authors to share articles published in our journal to other data platforms, but only if it is noted that it has been published in this journal.