Chitosan-based Nanosystems as Drug Carriers


  • R. Yu. Milusheva Institute of Polymer Chemistry and Physics, Academy of Sciences, Tashkent Kadyri 76, Tashkent, 100128, Uzbekistan
  • S. Sh. Rashidova Institute of Polymer Chemistry and Physics, Academy of Sciences, Tashkent Kadyri 76, Tashkent, 100128, Uzbekistan



The formation and application of polymeric nanomaterials is great demandin science, industry, biotechnology, and medicine due to the possibility ofachieving a significant improvement in the physicochemical, mechanical,and barrier properties of polymers and using them as drug carriers andfillers, which is especially promising for biodegradable polymers such aschitosan and their derivatives. The article presents methods for creatingpolymer nanostructures based on polysaccharides and, in particular,chitosan. Obtaining nanostructured samples of chitosan using theapproaches of chemical transformation and modification of polysaccharidesis an urgent scientific problem, the solution of which makes it possible toobtain new polymer systems of great practical interest. The medical aspectsof the use of polymer carriers based on chitosan for the treatment of variousdiseases are discussed. The unique specificity of the properties of chitosanand nanomaterials derived from it, with the properties inherent in thisnatural polymer, can serve as a promising future, especially in the field ofmedicine.


Chitosan; Nanochitosan; Modification; Nanoparticle synthesis; Chemical structure; Ionotropic gelation; Covalent crosslinking


[1] Birrenbach, G., Speiser, P.P., 1976. Polymerized micelles and their use as adjuvants in immunology. Journal of Pharmaceutical Sciences. 65, 1763-1766.

[2] Peer, D., Karp, J.M., Hong, S., et al., 2007. Nanocarriers as an emerging platform for cancer therapy. Nature nanotechnology. 2, 751-760.

[3] Adeli, M., Mirab, N., Zabihi, F., 2009. Nanocapsules based on carbon nanotubes-graft-polyglycerol hybrid materials. Nanotechnology. 20, 485603.

[4] Yi, H., Wu, L.Q., Bentley, W.E., et al., 2005. Biofabrication with chitosan. Biomacromolecules. 6, 2881- 2894.

[5] Aiba, S., 1992. Studies on chitosan: 4. Lysozymic hydrolysis of partially N-acetylated chitosans. International Journal of Biological Macromolecules. 14, 225-228.

[6] Zhang, H., Neau, S.H., 2002. In vitro degradation of chitosan by bacterial enzymes from rat cecal and colonic contents. Biomaterials. 23, 2761-2766.

[7] Escott, G.M., Adams, D.J., 1995. Chitinase activity in human serum and leukocytes. Infection and Immunity. 63, 4770-4773.

[8] Huang, M., Fong, C.W., Khor, E., et al., 2005. Transfection efficiency of chitosan vectors: effect of polymer molecular weight and degree of deacetylation. Journal of Controlled Release. 106, 391-406.

[9] Ozgel, G., Akbuga, J., 2006. In vitro characterization and transfection of IL-2 gene complexes. International Journal of Pharmacy. 315, 44-51.

[10] MacLaughlin, F.C., Mumper, R.J., Wang, J., et al., 1998. Chitosan and depolymerized chitosan oligomers as condensing carriers for in vivo plasmid delivery. Journal of Controlled Release. 56, 259-272.

[11] Richardson, S.C., Kolbe, H.V., Duncan, R., 1999. Potential of low molecular mass chitosan as a DNA delivery system: biocompatibility, body distribution and ability to complex and protect DNA. International Journal of Pharmacy. 178, 231-243.

[12] Borchard, G., 2001. Chitosans for gene delivery. Advanced Drug Delivery Reviews. 52, 145-150.

[13] Guliyeva, U., Oner, F., Ozsoy, S., et al., 2006. Chitosan microparticles containing plasmid DNA as potential oral gene delivery system. European Journal of Pharmaceutics and Biopharmaceutics. 62, 17-25.

[14] Mansouri, S., Lavigne, P., Corsi, K., et al., 2004. Chitosan-DNA nanoparticles as non-viral vectors in gene therapy: strategies to improve transfection efficacy. European Journal of Pharmaceutics and Biopharmaceutics. 57, 1-8.

[15] van der Lubben, I.M., Verhoef, J.C., Borchard, G., et al., 2001. Chitosan and its derivatives in mucosal drug and vaccine delivery. European Journal of Pharmaceutical Sciences. 14, 201-207.

[16] Sharma, S., Mukkur, T.K., Benson, H.A., et al., 2012. Enhanced immune response against pertussis toxoid by IgA-loaded chitosan-dextran sulfate nanoparticles. Journal of Pharmaceutical Sciences. 101, 233-244.

[17] Nam, J.P., Choi, C., Jang, M.K., et al., 2010. Insulin-incorporated chitosan nanoparticles based on polyelectrolyte complex formation. Macromolecular Research. 18, 630-635.

[18] Liu, Z., Jiao, Y., Liu, F., et al., 2007. Heparin/chitosan nanoparticle carriers prepared by polyelectrolyte complexation. Journal of Biomedical Materials Research Part A. 83A, 806-812.

[19] Janes, K.A., Fresneau, M.P., Marazuela, A., et al., 2001. Chitosan nanoparticles as delivery systems for doxorubicin. Journal of Controlled Release. 73, 255- 267.

[20] Crini, G., 2005. Recent developments in polysaccharide-based materials used as absorbents in waste water treatment. Progress in Polymer Science. 30, 38- 70.

[21] Peniche, H., Peniche, C., 2011. Chitosan nanoparticles: a contribution to nanomedicine. Polymer International. 60, 883-889.

[22] Huang, Y., Lapitsky, Y., 2011. Monovalent salt enhances colloidal stability during the formation of chitosan/tripolyphosphate microgels. Langmuir. 27, 10392-10399.

[23] Milusheva, R.Yu., Rashidova, S.Sh., 2022. Obtaining chitosan nanoparticles from Bombyx mori. Russian Chemical Bulletin, 71(2), 232-239.

[24] Rashidova, S.Sh., Milusheva, R.Yu., 2010. Nanostructured polysaccharides on the chitosan Bombyx mori base and possibility their using in medicine. 6th Nanofun-poly Conference. pр. 92. Madrid.

[25] Ivanushko, L.A., 2007. Comparative study of the immunomodulatory properties of chitosan and its derivatives. Medical Immunology. 9(4-5), 397-404.

[26] Carmen, R., Roland, L., 1997. Mechanical, water uptake and permeability properties of cross-linked chitosan glumate and alginate films. Journal of Controlled Release. 44, 215-225.

[27] Kadirova, D.A., Inoyatova, F.Kh., Baikulov, A.K., et al., 2022. Study of the binding of chitosan to specific DNA regions in the treatment of thermal burns. Bulletin of NGU. Series: Biology, clinical medicine. 10(5). 31-36. (Access on 20 May 2022).

[28] Pan, Y., Li, Y.J., Zhao, H.Y., et al., 2002. Bioadhesive polysaccharide in protein delivery system: chitosan nanoparticles improve the intestinal absorption of insulin in vivo. International Journal of Pharmacy. 249, 139-147.

[29] Lopez-Leon, T., Carvalho, E.L., Seijo, B., et al., 2005. Physicochemical characterization of chitosan nanoparticles: electrokinetic and stability behavior. Journal of Colloid and Interface Science. 283, 344- 351.

[30] Alishahi, A., Mirvaghefi, A., Tehrani, M.R., et al., 2011. Chitosan nanoparticle to carry vitamin C through the gastrointestinal tract and induce the non-specific immunity system of rainbow trout (Oncorhynchus mykiss). Carbohydrate Polymers. 86, 142-146.

[31] Alam, S., Khan, Z.I., Mustafa, G., et al., 2012. Development and evaluation of thymoquinone-encapsulated chitosan nanoparticles for nose-to-brain targeting: a pharmacoscintigraphic study. International Journal of Nanomedicine. 7, 5705-5718.

[32] Saha, P., Goyal, A.K., Rath, G., 2010. Formulation and evaluation of chitosan-based ampicillin trihydrate nanoparticles. Tropical Journal of Pharmaceutical Research. 9, 483-488.

[33] De Giglio, E., Trapani, A., Cafagna, D., et al., 2011. Dopamine-loaded chitosan nanoparticles: formulation and analytical characterization. Analytical and Bioanalytical Chemistry. 400, 1997-2002.

[34] Bellocq, A.M., Biais, J., Bothorel, P., et al., 1984. Microemulsions. Advances in Colloid and Interface Science. 20, 167-272.

[35] Banerjee, T., Mitra, S., Kumar Singh, A., et al., 2002. Preparation, characterization and biodistribution of ultrafine chitosan nanoparticles. International Journal of Pharmacy. 243, 93-105.

[36] You, J.O., Liu, Y.C., Peng, C.A., 2006. Efficient gene transfection using chitosan-alginate core-shell nanoparticles. International Journal of Nanomedicine. 1, 173-180.

[37] Manchanda, R., Nimesh, S., 2010. Controlled size chitosan nanoparticles as an efficient, biocompatible oligonucleotides delivery system. Journal of Applied Polymer Science. 118, 2071-2077.

[38] Sugimoto, M., Morimoto, M., Sashiwa, H., et al., 1998. Preparation and characterization of water-soluble chitin and chitosan derivatives. Carbohydrate Polymers. 36, 49-59.

[39] Gorochovceva, N., Makuška, R., 2004. Synthesis and study of water-soluble chitosan-O-poly(ethylene glycol) graft copolymers. European Polymer Journal. 40, 685-691.

[40] Vasquez, D., Milusheva, R., Baumann, P., et al., 2014. The amine content of PEGylated chitosan Bombyx mori nanoparticles acts as a trigger for protein delivery. Langmuir., 30(4), 965-975.

[41] Yang, X.D., Zhang, Q.Q., Wang, Y.S., et al., 2008. Self-aggregated nanoparticles from methoxy poly(ethylene glycol)-modified chitosan: Synthesis; characterization; aggregation and methotrexate release in vitro. Colloids and Surfaces B: Biointerfaces, B. 61(2), 125-131.

[42] Ouchi, T., Nishizawa, H., Ohya, Y., 1998. Aggregation phenomenon of PEG-grafted chitosan in aqueous solution. Polymer. 39(21), 5171-5175.

[43] Gan, Q., Wang, T., 2007. Chitosan nanoparticle as protein delivery carrier—Systematic examination of fabrication conditions for efficient loading and release. Colloids and Surfaces B: Biointerfaces. 59, 24-34.

[44] Moghaddam, F.A., Atyabi, F., Dinarvand, R., 2009. Preparation and in vitro evaluation of mucoadhesion and permeation enhancement of thiolated chitosan-pHEMA core-shell nanoparticles. Nanomedicine. 5, 208-215.

[45] Yu, R., Milusheva, O.B., Avazova, S.Sh., 2020. Rashidova Protein from pupae of the silkworm Bombyx mori L. Isolation, properties, application FAN, Tashkent. pp. 216. (Access on 20 May 2022)

[46] Schmitt, C., Sanchez, C., Desobry-Banon, S., et al., 1998. Structure and technofunctional properties of protein-polysaccharide complexes: A review. Critical Reviews in Food Science and Nutrition. 38, 689-753.

[47] Jayakumar, R., Menon, D., Manzoor, K., et al., 2010. Biomedical applications of chitin and chitosan based nanomaterials—A short review. Carbohydrate Polymers. 8.

[48] Zhang, L., Zhao, Z.L., Wei, X.H., et al., 2013. Preparation and in vitro and in vivo characterization of cyclosporin A-loaded, PEGylated chitosan-modified, lipid-based nanoparticles. International Journal of Nanomedicine. 8, 601-610.

[49] Chadha, R., Bhandari, S., Kataria, D., et al., 2012. Exploring the potential of lecithin/chitosan nanoparticles in enhancement of antihypertensive efficacy of hydrochlorothiazide. Journal of Microencapsulation. 29, 805-812.

[50] Bhattarai, N., Ramay, H.R., Chou, S.H., et al., 2006. Chitosan and lactic acid-grafted chitosan nanoparticles as carriers for prolonged drug delivery. International Journal of Nanomedicine. 1, 181-187.

[51] Gregorio-Jauregui, K.M., Pineda, M.G., Rivera-Salinas, J.E., et al., 2012. One-step method for preparation of magnetic nanoparticles coated with chitosan. Journal of Nanomaterials. 8.

[52] Dorniani, D., Hussein, M.Z., Kura, A.U., et al., 2013. Preparation and characterization of 6-mercaptopurine-coated magnetite nanoparticles as a drug delivery system. Drug Design, Development and Therapy. 7, 1015-1026.

[53] R.Yu. Milusheva, S.Sh., 2017. Rashidova Bioactive properties of nanochitosan Bombyx mori. Polymer Science, Series C. 59, 29–34.

[54] Hu, Y., Jiang, X., Ding, Y., et al., 2002. Synthesis and characterization of chitosan-poly(acrylic acid) nanoparticles. Biomaterials. 23, 3193-3201.

[55] Gazori, T., Khoshayand, M.R., Azizi, E., et al., 2009. Evaluation of Alginate/Chitosan nanoparticles as antisense delivery vector: formulation, optimization and in vitro characterization. Carbohydrate Polymers. 77, 599-606.

[56] Zheng, F., Shi, X.W., Yang, G.F., et al., 2007. Chitosan nanoparticle as gene therapy vector via gastrointestinal mucosa administration: results of an in vitro and in vivo study. Life Sciences. 80, 388-396.


How to Cite

Milusheva, R. Y., & Rashidova, S. S. (2022). Chitosan-based Nanosystems as Drug Carriers. Organic Polymer Material Research, 4(1), 24–37.


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