Isolation and Identification of the Ammonium and Nitrite Oxidizing Bacterial Strains for Nitrogen Treatment in Shrimp Pond in Quang Ninh Province, Vietnam

Authors

  • Nguyen Thi Tham

    Ha Long University, 258 Bach Dang street, Nam Khe ward, Uong Bi, 200000, Quang Ninh province, Vietnam
  • Pham Quy Giang

    Ha Long University, 258 Bach Dang street, Nam Khe ward, Uong Bi, 200000, Quang Ninh province, Vietnam
  • Dinh Quynh Oanh

    Ha Long University, 258 Bach Dang street, Nam Khe ward, Uong Bi, 200000, Quang Ninh province, Vietnam
  • Diep Thi Thu Thuy

    Ha Long University, Vietnam
  • Hoang Thi Bich Hong

    Ha Long University, 258 Bach Dang street, Nam Khe ward, Uong Bi, 200000, Quang Ninh province, Vietnam
  • Pham Thi Huong

    Ha Long University, 258 Bach Dang street, Nam Khe ward, Uong Bi, 200000, Quang Ninh province, Vietnam

DOI:

https://doi.org/10.30564/jees.v6i2.6749
Received: 14 June 2024 | Revised: 24 June 2024 | Accepted: 30 June 2024 | Published Online: 20 July 2024

Abstract

The objective of this study was to isolate and identify the nitrifying bacteria from wastewater in the aquaculture system at Quang Ninh province and initially evaluate their ability to treat nitrogen in water and determine the strain of bacteria and identify the strain of bacteria. Nitrifying bacteria were isolated by serial dilution and agar plates and then purified by agar slant until axenic colonies were obtained. The study has successfully isolated 3 bacterial strains capable of ammonium oxidation (AOB-1, AOB-2, AOB-3) and 3 bacterial strains with nitrite oxidation ability (NOB-1, NOB-2, NOB-3) from shrimp farming wastewater in Quang Ninh province, Vietnam. These strains demonstrate potential for ammonium and nitrite oxidation, making them applicable for nitrogen treatment in sewage. Strains with nitrite oxidation ability showed a potential high capability for nitrogen removal from water. The decoding of the 16S rRNA gene sequences, compared using Blastn (NCBI) indicated similarities with Bacillus subtilis (AOB-1), Pantoea agglomerans strain JCM1236 16S RNA (AOB-2), Enterobacter hormaechei strain ES1 16S RNA (AOB-3), Arthrobacter nicotianae (NOB-1), Acinetobacter lactucae strain JVAP01 16S RNA (NOB-2) and Enterobacter asburiae (NOB-3). Therefore, the bacteria that contained 6 strains can be considered to apply for wastewater treatment in shrimp farming.

Keywords:

Bacteria isolation; Identification; Nitrification; Eutrophication; Ammonium oxidizing; Nitrite oxidizing

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How to Cite

Nguyen Thi Tham, Pham Quy Giang, Dinh Quynh Oanh, Diep Thi Thu Thuy, Hoang Thi Bich Hong, & Pham Thi Huong. (2024). Isolation and Identification of the Ammonium and Nitrite Oxidizing Bacterial Strains for Nitrogen Treatment in Shrimp Pond in Quang Ninh Province, Vietnam. Journal of Environmental & Earth Sciences, 6(2), 187–199. https://doi.org/10.30564/jees.v6i2.6749

Issue

Vol. 6 , Iss. 2 (July 2024)

Article Type

Article

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Copyright © 2024 Author(s)

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This is an open access article under the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) License.

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