Studying, Analyzing, and Interpreting the Gut Microbiome of the Earthworm M. peguana (Rosa, 1890) Using Next-Generation Sequencing

Abstract

This study investigates the diversity of gut microbiota in Metaphire peguana, an earthworm species commonly found in agricultural areas of Thailand. Earthworms play a critical role in soil ecosystems by supporting nutrient cycling and breaking down organic matter. Understanding the microbial diversity in their gut is essential for exploring their ecological contributions. Using Next Generation Sequencing (NGS), we analyzed the mycobiome in the gut of M. peguana. Our findings revealed a high diversity of fungal species, primarily belonging to two major phyla: Ascomycota and Basidiomycota. Ascomycota was the most abundant phylum, comprising 40.1% of the total fungal species identified. A total of 33 distinct fungal species were identified, which underscores the richness of microbial life within the earthworm gut. This study successfully created the first genetic database of the microbial community in M. peguana, providing a foundation for future research in agricultural applications. The microbial species identified, particularly siderophore-producing fungi, could have significant implications for improving soil fertility and promoting sustainable agricultural practices. The use of NGS technology has enabled comprehensive profiling of microbial communities, allowing for precise identification of fungi that may play essential roles in soil health. Furthermore, the study paves the way for future studies on the potential applications of earthworm gut microbiomes in biotechnology, especially in enhancing soil nutrient availability and plant growth. The findings of this research contribute to the broader understanding of the ecological roles of earthworms and their microbiomes in soil ecosystems.