Legume Green Manuring Improves Soil Fertility and Plant Growth of Eucalyptus Plantation in South Subtropical China


  • Kongxin Zhu Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China
  • Huili Wang Academy of Forestry Research of Guangxi Autonomous Region, Nanning, 530002, China
  • Zuoyu Qin Academy of Forestry Research of Guangxi Autonomous Region, Nanning, 530002, China
  • Jian Tang Academy of Forestry Research of Guangxi Autonomous Region, Nanning, 530002, China
  • Xiaojun Deng Academy of Forestry Research of Guangxi Autonomous Region, Nanning, 530002, China
  • Jizhao Cao Academy of Forestry Research of Guangxi Autonomous Region, Nanning, 530002, China
  • Shunyao Zhuang Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China




Legume green manure is extensively planted to improve soil fertility in crop field. However, the application of legume in Eucalyptus plantation is still limited and depends on site specific and species. Therefore, the objective of this study was to determine the effects of green manure interplantation on soil fertility and plant growth of Eucalyptus plantation in a short term. A field experiment of one year was established to investigate the green manure growth, forest soil nutrients and Eucalyptus plant growth inter-planted with two legume species (Tephrosia candida, TC and Sesbania cannabina, SC) at south subtropical China. Legumes were inter-planted in linear among the tree space of Eucalyptus stand. Result showed that the green manure inter-plantation increased soil organic matter by 9.66% of TC and 18.44% of SC. Soil available nitrogen, phosphorus and potassium were improved significantly by the legume treatments as well. The increment of height and diameter at breast height of Eucalyptus during the experiment was significant in legume treatments. Thus, the timber volume increment was improved significantly by 46.81% of TC and 35.47% of SC compared with the control treatment. Therefore, the inter-plantation of legume green manure under the Eucalyptus plantation is effective to improve soil fertility and tree growth. Such a measure is potential and referenced for the sustainable forest management.


Eucalyptus plantation, Green manure, Legume plant, Soil fertility


[1] SFAPRC (State Forestry Administration, P.R. China). Statistics of forest resources in China. 2018. (in Chinese)

[2] Binkley, D., Campoe, O.C., Alvares, C., Carneiro, R.L., Cegatta, Í., Stape, J.L.. The interactions of climate, spacing and genetics on clonal Eucalyptus plantations across Brazil and Uruguay. Forest Ecology & Management, 2017, 405, 271-283. DOI: https://doi.org/10.1016/j.foreco.2017.09.050.

[3] McMahon, D.E., Vergutz, L., Valadares, S.V., Silva, I.R., Jackson, R.B.. Soil nutrient stocks are maintained over multiple rotations in Brazilian Eucalyptus plantations. Forest Ecology & Management, 2019, 448: 364-375. DOI: https://doi.org/10.1016/j.foreco.2019.06.027.

[4] Ma, Q., Yu, W.T., Shen, S.M., Zhou, H., Jiang, Z.S., Xu, Y.G.. Effects of fertilization on nutrient budget and nitrogen use efficiency of farmland soil under different precipitations in Northeastern China. Nutrient Cycling in Agroecosystem. 2010, 88, 315-327. DOI: https://doi.org/10.1007/s10705-010-9356-6.

[5] Silva, P.H.M. da, Poggiani, F., Libardi, P.L., Gonçalves, A.N.. Fertilizer management of eucalypt plantations on sandy soil in Brazil: Initial growth and nutrient cycling. Forest Ecology & Management. 2013, 301, 67–78. DOI: https://doi.org/10.1016/j.foreco.2012.10.033.

[6] Laganie `re, J., Angers, D.A., Pare, D.. Carbon accumulation in agricultural soils after afforestation: a meta-analysis. Global Change Biology. 2010,16(1):439-453. DOI: https://doi.org/10.1111/j.1365-2486.2009.01930.x.

[7] Fialho, R.C., Zinn, Y.L.. Changes in soil organic carbon under eucalyptus plantations in Brazil: a comparative analysis. Land Degradation & Development. 2014,25(5):428-437. DOI: https://doi.org/10.1002/ldr.2158.

[8] Li, X., Ye, D., Liang, H., Zhu, H., Qin, L., Zhu,Y., Qin, L., Zhu, Y., Wen, Y.. Effects of successive rotation regimes on carbon stocks in Eucalyptus plantations in subtropical China measured over a full rotation. PLoS ONE. 2015, 10(7):e0132858. DOI: https://doi.org/10.1371/journal.pone.0132858.

[9] Goncalves, J.L.M., Stape, J.L., Laclau, J.P., Smethurst, P., Gava, J.L.. Silvicultural effects on the productivity and wood quality of eucalypt plantations. Forest Ecology & Management. 2004, 193: 45–61. DOI: https://doi.org/10.1016/j.foreco.2004.01.022.

[10] Liao, G.R.. Review of researches on soil problems of eucalyptus plantation sites in China. Ecology and Environment. 2003, 12(1): 119-121. (in Chinese)

[11] Xie. Z.J., Tu, S.X., Shah, F., Xu, C.X., Chen, J.R., Han, D., Liu, G.R., Li, H.L., Muhammad, I., Cao, W.D.. Substitution of fertilizer-N by green manure improves the sustainability of yield in double-rice cropping system in south China. Field Crop Research. 2016, 188: 142–149. DOI: https://doi.org/10.1016/j.fcr.2016.01.006.

[12] Astier, M., Maass, J.M., Etchevers-Barra, J.D., Peña, J.J., de León González, F.. Short-term green manure and tillage management effects on maize yield and soil quality in an Andisol. Soil and Tillage Research. 2006, 88(1-2), 153-159. DOI: https://doi.org/10.1016/j.still.2005.05.003.

[13] Yusuf, A.A., Abaidoo, R.C., Iwuafor, E.N.O., Olufajo, O.O., Sanginga, N.. Rotation effects of grain legumes and fallow on maize yield, microbial biomass and chemical properties of an Alfisol in the Nigerian savanna. Agriculture, Ecosystem & Environment. 2009, 129: 325-331. DOI: https://doi.org/10.1016/j.agee.2008.10.007.

[14] Zhou, G.P., Cao, W.D., Bai, J.S., Xu, C.G., Zeng, N.H., Gao, S.J., Rees, R.M., Dou, F.G.. Co-incorporation of rice straw and leguminous green manure can increase soil available nitrogen (N) and reduce carbon and N losses: An incubation study. Pedosphere. 2020, 30(5): 661-670. DOI: https://doi.org/10.1016/S1002-0160(19)60845-3.

[15] Lin, Z.M., Hou, Q.Z., Luo, G.Y., Dong, C.H., Mai, R.Z., Chen, S.G.. Interplanting Desmodium heterocarpon var. strigosum Meeuwen under Eucalyptus plantations. Guangdong Agricultural Science. 2014,44(12): 45-52. (in Chinese)

[16] Gao, W.J., Cheng, F., Lin, J.P., Cai, Q.X., Liu, A.Q.. Effects of interplanting green manure on soil physical and chemical properties of eucalyptus plantation. Journal of Northwest Forestry University. 2019, 34(1): 54-61. (in Chinese)

[17] Lu, R,K., Analytical methods of soil agrochemistry. Beijing: China Agricultural Science and Technology Press. 2000. (in Chinese).

[18] Lopez, D.M.S., Arturi, M.F., Goya, J.F., Perez, C.A., Frangi, J.L.. Eucalyptus grandis plantations: effects of management on soil carbon, nutrient contents and yields. Journal of Forest Research. 2020, 31(2): 601- 611. DOI: https://doi.org/10.1007/s11676-018-0850-z.

[19] Thornley, J.H.M., Cannell, M.G.R.. Managing forests for wood yield and carbon storage: a theoretical study. Tree Physiology. 2000, 20:477–484. DOI: https://doi.org/10.1093/treephys/20.7.477.

[20] Jandl, R., Lindner, M., Vesterdal, L., Bauwens, B., Baritz, R., Hagedorn, F., Johnson, D.W., Minkkinen, K., Byrne, K.A.. How strongly can forest management influence soil carbon sequestration? Geoderma. 2007, 137(3):253-268. DOI: https://doi.org/10.1016/j.geoderma.2006.09.003.

[21] Mendham, D.S., Heagney, E.C., Corbeels, M., O’Connell, A.M., Grove, T.S., McMurtrie, R.E.. Soil particulate organic matter effects on nitrogen availability after afforestation with Eucalyptus globulus. Soil Biology & Biochemistry. 2004, 36(7):1067- 1074. DOI: https://doi.org/10.1016/j.soilbio.2004.02.018.

[22] Li, X.Q., Ye, D., Liang, H.W., Zhu, H.G., Qin, L., Zhu, Y.L., Wen, Y.G.. Effects successive rotation rgeimes on carbon stocks in Eucalyptus plantations in subtropical China measured over a full rotation. PLoS ONE, 2015 e0321858. DOI: https://doi.org/10.1371/journal.pone.0132858.

[23] Liang, L.N., Gui, X.G., Liao, X., Qin, L., Liao, H.. Screening and preliminary application of rapeseed materials as green manure intercropped in tea plantations. Chinese Journal of Oil Crop Sciences. 2019, 41(6): 825-834 (in Chinese)

[24] Liu, X.F., Liu, C.Z., Wang, S.G., Li, B.Y.. Effects of green manure on soil nutrients, aggregation, and distributions of carbon and nitrogen. Tianjin Agricultural Sciences. 2015, 21(8): 44-47 (in Chinese)

[25] Zhu, X.M., Wen, Z.G., Zhao, B.Q., Liu, C., Xin, J.C., Dong, J., Ding, H.R., Hong, L.Z.. Effects of planting green manure on dynamic changes of saline soil nutrients and soluble salt ions. Southwest China Journal of Agricultural Sciences. 2017, 30(8): 1894-1898 (in Chinese)

[26] Fungo, B., Lehmann, J., Kalbitz, K.. Emissions intensity and carbon stocks of a tropical Ultisol after amendment with Tithonia green manure, urea and biochar. Field crops research, 2017, 209. DOI: https://doi.org/10.1016/j.fcr.2017.05.013.

[27] Li, Z., Liu, G.S., Jin, H.X., Ye, X.F., Xie, C.S., Xiang,Y.G., Zhang, W.P., Yang, C., Wang, Y., Xi, X.Y.. Effects of green manure application combined with chemical fertilizers on microbial biomass C, N and nitrogen sypplying characteristics of tobacco-planting soils. Acta Prataculturea Sinica. 2011,20(6): 126-134. DOI: https://doi.org/1004-5759(2011)20:62.0.TX;2-8.

[28] Zhu, B., Yi, L.X., Hu, Y.G., Zeng, Z.H., Lin, C.W., Tang, H.M., Yang, G.L., Xiao, X.P.. Nitrogen release from incorporated 15N-labelled Chinese milk vetch (L.) residue and its dynamics in a double rice cropping system. Plant Soil. 2014,(374):331-344. DOI: https://doi.org/10.1007/s11104-013-1808-8.

[29] Wang, D.K., Yu, X.Y., Zhang, X.F., Huang, L., Li, X.T., He, Z.B., Kang, L., Wang, D.J., Yao, L.H., Guo, Y.J.. Responses of seed germination and rhizobia antioxidative enzyme activities in legume to acidity and aluminum and NaCl stresses. Acta Prataculturae Sinca. 2018,27(10): 35-44. (in Chinese)

[30] Du, Q.F., Wang, D.J., Yu, X.Y., Yao, L.H., He, Y.J., Wang, R., Ma, S.L., Guo, Y.J.. The effects of corn and green manure intercropping on soil nutrient availability and plant nutrient uptake. Acta Praaculturea Sinica, 2016, 25(3): 255-233. (in Chinese)

[31] Xie, W.J., Wang, J.S., Jin, X.X., Zhang, Y.P., Wu, L.F., Ouyang. Z.. Effect of Sesbania cannabina Cultivation on Severe Salinity Soil Fertility Improvement. Chinese Agricultural Science Bulletin. 2016, 32(6) :11- 123. (in Chinese)

[32] Yang, L., Wang, T., Xia, D.. Effects of intercropping with post-grafting generation of Impatiens balsamina on potassium uptake of grape seedlings under cadmium stress. IOP Conference Series: Earth and Environmental Science, 2019, 267(5). DOI: https://doi.org/10.1088/1755-1315/267/5/052025.

[33] Wang, L.Y., Xiao, H., Chen, W.J., Zhao, J., Wang, X.F., Pan, J.. Effects of Different Fertilization Mode on Crops Yield and Soil Fertility in Coastal Saline Soil. Acta Agriculture Boreali-Sinica. 2016, 31(5): 222-227. (in Chinese)

[34] Qin, J.H., He, H.Z., Li, H.S., Gu, C., Peng, Y.Y., Han, M.. Allelopathic Effect of Returning Plant Residue to Field from Sesame, Peanut and Sesbania. Journal of Agro-Environment Science. 2012, 31(10): 1941- 1947. (in Chinese)

[35] Bai, C.J., Yu, D.G., Chen, Z.Q., Liu, G.D.. Impact of silvopastoral systems on growth and litter amount of Eucalyptus AbL 12 Plantation. Chinese Journal of Tropical Crops. 2012, 33(10): 1896-1902. (in Chinese)

[36] Preethi, B., Poorniammal, R., Balachandar, D., Karthikeyan, S., Chendrayan, K., Bhattacharyya, P., Adhya T.K.. Long-term organic nutrient managements foster the biological properties and carbon sequestering capability of a wetland rice soil. Archives of Agronomy and Soil Science. 2013, 59(12): 1607- 1624. DOI: https://doi.org/10.1080/03650340.2012.755260.


How to Cite

Zhu, K., Wang, H., Qin, Z., Tang, J., Deng, X., Cao, J., & Zhuang, S. (2021). Legume Green Manuring Improves Soil Fertility and Plant Growth of Eucalyptus Plantation in South Subtropical China. Research in Ecology, 3(1), 14–21. https://doi.org/10.30564/re.v3i1.2637


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