Isolation and Characterization of Plant Growth Promoting Endophytes from Linum Usitatissimum

Authors

  • Shalu Choudhary

    Himalayan School of Biosciences, Swami Rama Himalayan University, Jolly Grant, Dehradun, Uttarakhand 248140, India
  • Geeta Bhandari

    Himalayan School of Biosciences, Swami Rama Himalayan University, Jolly Grant, Dehradun, Uttarakhand 248140, India
  • Anant Deogaonkar

    Symbiosis Institute of Business Management, Nagpur, Symbiosis International (Deemed) University, Pune 440008, India
  • Deepshree Kumar

    Ramdeobaba University, School of Management, Nagpur 440013, India
  • Kanishka Miglani

    Himalayan School of Biosciences, Swami Rama Himalayan University, Jolly Grant, Dehradun, Uttarakhand 248140, India
  • Sanjay Gupta

    Himalayan School of Biosciences, Swami Rama Himalayan University, Jolly Grant, Dehradun, Uttarakhand 248140, India
  • Samiksha Joshi

    Himalayan School of Biosciences, Swami Rama Himalayan University, Jolly Grant, Dehradun, Uttarakhand 248140, India
  • Amit Mittal

    Department of Allied Sciences, Graphic Era Hill, University, Bhimtal 263136, India
  • Saurabh Gangola

    Department of Microbiology, Graphic Era Deemed to be University, Dehradun 248002, India

DOI:

https://doi.org/10.30564/re.v7i2.9406
Received: 7 April 2025 | Revised: 6 May 2025 | Accepted: 15 May 2025 | Published Online: 30 May 2025

Abstract

This present study identifies endophytic bacteria from Linum usitatissimum with multidimensional plant growth-promoting attributes, positioning them as ecological engineers for sustainable agriculture. Plant growth-promoting bacteria (PGPB) are present in symbiotic associations with plants or rhizosphere. These microbes enhance crop productivity and resilience under different environmental conditions. Endophytes are a type of PGPB that inhabit inside plant tissues and contribute to plant growth by phytohormone production, phosphate solubilisation, zinc solubilisation, siderophore production, ammonia production, nitrogen fixation, stress tolerance, and biocontrol mechanisms. Twelve bacterial strains were isolated from Linum usitatissimum exhibiting plant growth-promoting attributes such as ammonia and indole-3-acetic acid (IAA) production, siderophore synthesis, phosphate solubilisation, and extracellular enzyme synthesis. The isolated endophytes were also assessed for different enzymatic activities such as; cellulase, pectinase, xylanase, amylase, and gelatinase, which contribute to development of a symbiotic relationship and are crucial for the degradation of plant cell wall components The most efficient endophytes identified in the present study were Pseudomonas sp. strain JL-1 (ESL1) and Staphylococcus sciuri (ESL2), both of which displayed strong plant growth-promoting potential. ESL1 and ESL2 demonstrated promising plant growth-promoting characteristics and cellulase, pectinase, xylanase, amylase, and gelatinase, activity. ESL2 (Staphylococcus sciuri) enhanced nutrient cycling (phosphate solubilisation: 196–209 µg/ml; siderophores: 68–71%) and stress tolerance (IAA: 11–12 µg/ml), reducing reliance on synthetic inputs.  By integrating flax microbiomes into agro-ecosystems, we demonstrate a scalable approach to reconcile crop productivity with soil biodiversity conservation. These results demonstrate the potentiality of these endophytic microbes in sustainable agriculture, environmental management, and microbial biotechnology. Further studies on their metabolic pathways may expand their applications in bioremediation and plant-microbe interactions.

Keywords:

Linum usitatissimum; Bacteria; Plant Growth; Biochemical; Siderophore

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How to Cite

Shalu Choudhary, Bhandari, G., Anant Deogaonkar, Deepshree Kumar, Kanishka Miglani, Sanjay Gupta, Samiksha Joshi, Amit Mittal, & Gangola, S. (2025). Isolation and Characterization of Plant Growth Promoting Endophytes from Linum Usitatissimum. Research in Ecology, 7(2), 129–143. https://doi.org/10.30564/re.v7i2.9406

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Vol. 7, Iss. 2 (June 2025) (in progress)

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Copyright © 2025 Shalu Choudhary, Geeta Bhandari, Anant Deogaonkar, Deepshree Kumar, Kanishka Miglani, Sanjay Gupta, Samiksha Joshi, Amit Mittal, Saurabh Gangola

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This is an open access article under the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) License.

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