Bacterial Microflora on Freshwater Prawn (Macrobrachium rosenbergii) and Culture Water Associated with Public Health Concern

Authors

  • Md. Rayhan Ali Blue Gold Program, Bangladesh Water Development Board, Khulna -9100, Bangladesh
  • Nipa Gupta Department of Aquaculture, Hajee Mohammad Danesh Science & Technology University, Dinajpur-5200, Bangladesh
  • S.M. Shahjahan Siraj Department of Fisheries, Ministry of Fisheries and Livestock, Bangladesh
  • Mousumi Sarker Chhanda Department of Aquaculture, Hajee Mohammad Danesh Science & Technology University, Dinajpur-5200, Bangladesh
  • Md. Nazmul Ahsan Fisheries and Marine Resource Technology Discipline, Khulna University, Khulna, Bangladesh

DOI:

https://doi.org/10.30564/re.v1i2.973

Abstract

Quantitative and qualitative analyses of important bacterial content for public health concern (total bacterial count, total coliforms, faecal coliforms, Salmonella and Vibrio cholerae) of  cultured fresh water prawn and  farm water which has significant role in order to manage sustainable aquaculture were carried out. Microbiological parameters of prawn (Macrobrachium rosenbergii) and farm water were determined by following the ISO standard methods. Total bacterial Count (standard plate count) found in prawn samples ranged from 5.55 to 5.71 log CFU/g while 4.13 to 4.18 log CFU/mL in water sample. On the other hand, total coliforms found in prawn sample ranged between 1.96 to 2.46 log CFU/g whereas in water sample 2.07 to 2.46 log CFU/mL total coliforms were detected.  In case of faecal coliforms, the number ranged between 0.96 to 1.42 log CFU/g in prawn sample and 1.59 to 1.81 in water sample. While Vibrio cholerae was absent in both prawns and water sample and Salmonella was detected in two tested ponds for both prawn and water sample. 

Keywords:

M. rosenbergii; Farm water; Bacterial load; Salmonella

References

[1] Ahmed, N., H. Demaine, and J.F. Muir, Freshwater prawn farming in Bangladesh: history, present status and future prospects. Aquaculture Research, 2008. 39(8): 806-819.

[2] Wahab, M.A., et al., Current status and prospects of farming the giant river prawn M acrobrachium rosenbergii (D e M an) in B angladesh. Aquaculture Research, 2012. 43(7): 970-983.

[3] Ahmed, N., J.H. Brown, and J.F. Muir, Freshwater prawn farming in gher systems in southwest Bangladesh. Aquaculture Economics & Management, 2008. 12(3): 207-223.

[4] Alam, R. and H. Demaine, Integrated prawn farming systems of southeast Bangladesh. Noakhali, Bangladesh: Ministry of Fisheries and Livestock, Bangladesh, 2004.

[5] Ahmed, N., Socio-economic aspects of freshwater prawn culture development in Bangladesh. 2001.

[6] DoF, Fishery Statistical Yearbook of Bangladesh 2004-2005. Department of Fisheries, Ministry of Fisheries and Livestock Dhaka, 2006.

[7] Kennedy, B., et al., Bacterial flora associated with the giant freshwater prawn Macrobrachium rosenbergii, in the hatchery system. Aquaculture, 2006. 261(4): 1156-1167.

[8] Anderson, I., M.N. Shamsudin, and G. Nash, A preliminary study on the aerobic heterotrophic bacterial flora in giant freshwater prawn, Macrobrachium rosenbergii, hatcheries in Malaysia. Aquaculture, 1989. 81(3-4): 213-223.

[9] Miyamoto, G., et al. A preliminary microbiological and water quality survey of two Hawaiian prawn (Macrobrachium rosenbergii) hatcheries. in Proceedings of the First International Conference onWarmwater Aquaculture, Crustacea (ed. by GL Roger, R. Day & A. Lim), 1983.

[10] Khondaker, H. Freshwater prawn resources of Bangladesh and role of department of fisheries for prawn farming development. in Abstracts-national workshop on freshwater prawn farming: search for new technologies, Bangladesh Agricultural University and Bangladesh Fisheries Research Forum, Mymensingh, Bangladesh, 2007.

[11] Muir, J., The future for fisheries: livelihoods, social development and environment, Fisheries Sector Review and Future Development Study. Commissioned with the association of the World Bank, DANIDA, USAID, FAO, DFID with the cooperation of the Bangladesh Ministry of Fisheries and Livestock and the Department of Fisheries, Dhaka, 2003. 81.

[12] Muir, J., The future for fisheries: economic performance. Fisheries Sector Review and Future Development Study. Commissioned with the association of the World Bank, DANIDA, USAID, FAO, DFID with the cooperation of the Bangladesh Ministry of Fisheries and Livestock and the Department of Fisheries, Dhaka, 2003.

[13] Ahmed, N., E.H. Allison, and J.F. Muir, Rice fields to prawn farms: a blue revolution in southwest Bangladesh? Aquaculture International, 2010. 18(4): 555-574.

[14] Deb, A.K., Fake blue revolution: environmental and socio-economic impacts of shrimp culture in the coastal areas of Bangladesh. Ocean & Coastal Management, 1998. 41(1): 63-88.

[15] Reilly, P. and D. Twiddy, Salmonella and Vibrio cholerae in brackishwater cultured tropical prawns. International journal of food microbiology, 1992. 16(4): 293-301.

[16] Ghosh, A.K., et al., Prevalence of coliform and Salmonella in cultured prawn (Macrobrachium rosenbergii) ghers of different management conditions at Dumuria, Khulna, Bangladesh, 2009.

[17] Brock, J.A., A synopsis of pathology, diseases, and production problems of cultured Macrobrachium, with an emphasis on experiences in Hawaiian prawn farming. CRC handbook of mariculture, 1993. 1: 361e391.

[18] Jayasree, L., P. Janakiram, and R. Madhavi, Shell disease in the freshwater Prawn Macrobrachium rosenbergii (de Man): Aetiology, Pathogenicity and Antibiotic sensitivity. Journal of Aquaculture in the Tropics, 1999.

[19] Maturin, L. and J. Peeler, Aerobic platecount. In, bacteriological Analytical Manual, 2011, 3.

[20] Andrews, W. and T. Hammack, Food sampling and preparation of sample homogenate. Bacteriological Analytical Manual, 2003: 1-10.

[21] Collins, C., P.M. Lyne, and J. Grange, Microbiological methods. Chrchill L" Medical microbiology". DG Turk and IA Po'l, ter a short text book of medical microbiology, 1985.

[22] Feng, P., et al., Enumeration of Escherichia coli and the coliform bacteria. Bacteriological Analytical Manual. Food and drug Administration (FDA) US department of Health & Human service, 2002.

[23] Oblinger, J. and J. Koburger, Understanding and teaching the most probable number technique. Journal of Milk and Food technology, 1975. 38(9): 540-545.

[24] Lalitha, K.V. and P.K. Surendran, Bacterial microflora associated with farmed freshwater prawn Macrobrachium rosenbergii (de Man) and the aquaculture environment. Aquaculture Research, 2004. 35(7): 629-635.

[25] Buchanan, R., R. Buchanan, and N. Gibbons, Bergey's Manual of Determinative Bacteriology. 1970: William's and Wilkins Company.

[26] Horsley, R., The bacterial flora of the Atlantic salmon (Salmo salar L.) in relation to its environment. Journal of Applied Bacteriology, 1973. 36(3): 377-386.

[27] Austin, B. and D. Allen‐Austin, Microbial quality of water in intensive fish rearing. Journal of applied bacteriology, 1985. 59: 207S-226S.

[28] Gonzalez, C.-J., et al., Bacterial microflora of wild brown trout (Salmo trutta), wild pike (Esox lucius), and aquacultured rainbow trout (Oncorhynchus mykiss). Journal of Food Protection, 1999. 62(11): 1270-1277.

[29] Surendran, P., N. Thampuran, and K. Gopakumar, Microbial profile of cultured fishes and prawns viz a viz their spoilage and contamination. FAO Fisheries Report, 1995(514 supplement): 1-12.

[30] Surendran, P., N. Thampuran, and V. Nambiar, Comparative Microbial Ecology of Fresh Water and Brackish Water Prawn Farms, 2000.

[31] Lalitha, K., K. Gopakumar, and P. Matsyapuri. Occurrence of Clostridium botulinum in farmed fish and shrimp in India. in Summary Report of and Papers Presented at the Tenth Session of the Working Party on Fish Technology and Marketing: Colombo, Sri Lanka, 4-7 June 1996. Food & Agriculture Organization of the UN (FAO), 1997.

[32] Uddin, M.N. and A. Al-Harbi, Quantitative and qualitative bacterial flora of giant freshwater prawn Macrobrachium rosenbergii cultured in earthen ponds in Saudi Arabia. Journal of Aquatic Animal Health, 2005. 17(3): 244-250.

[33] Reilly, A., M. Bernarte, and A. Dangla, Quality changes in brackish water praws ( Penaeus monodon) during storage at ambient temperature, in ice and after delays in icing. Spoilage of tropical fi sh and product development, 1985: 71.

[34] Gennari, M., S. Tomaselli, and V. Cotrona, The microflora of fresh and spoiled sardines (Sardina pilchardus) caught in Adriatic (Mediterranean) Sea and stored in ice. Food Microbiology, 1999, 16(1): 15-28.

[35] Jayaprakash, N., et al., Vibrios associated with Macrobrachium rosenbergii (De Man, 1879) larvae from three hatcheries on the Indian southwest coast. Aquaculture Research, 2006, 37(4): 351-358.

Downloads

How to Cite

Ali, M. R., Gupta, N., Shahjahan Siraj, S., Chhanda, M. S., & Ahsan, M. N. (2019). Bacterial Microflora on Freshwater Prawn (Macrobrachium rosenbergii) and Culture Water Associated with Public Health Concern. Research in Ecology, 1(2), 31–44. https://doi.org/10.30564/re.v1i2.973

Issue

Article Type

Articles