Phytochemical Study and Anti-nutritional Factors in Stems of Dioscorea praehensilis Benth (Dioscoreaceae)

Authors

  • Patience Mpia Ngelinkoto Department of chemistry and environment, Faculty of Sciences, National Pedagogic University, Kinshasa, Democratic Republic of Congo
  • André-Marie Kassia Lokassa Department of chemistry and environment, Faculty of Sciences, National Pedagogic University, Kinshasa, Democratic Republic of Congo
  • Bernadin Bulumuka Department of chemistry and environment, Faculty of Sciences, National Pedagogic University, Kinshasa, Democratic Republic of Congo
  • Jeff Kawaya Maliani Department of chemistry and environment, Faculty of Sciences, National Pedagogic University, Kinshasa, Democratic Republic of Congo
  • Myriam Mukadi Ngondo Department of Biology, Faculty of Sciences, National Pedagogic University, Kinshasa, Democratic Republic of Congo
  • Ruth Luntadila Mbuli Catholic University of the Congo, Kinshasa, Democratic Republic of Congo
  • Johnny Bopopi Mukoko Department of Biology, Faculty of Sciences, National Pedagogic University, Kinshasa, Democratic Republic of Congo
  • Florent Biduaya Mukeba Department of Biology, Faculty of Sciences, National Pedagogic University, Kinshasa, Democratic Republic of Congo

DOI:

https://doi.org/10.30564/jbr.v3i4.3455

Abstract

The aim of this research was to find and assay phytochemical compounds and various biological macromolecules of the tender stems of Dioscorea praehensilis benth and evaluate their antioxidant activity and to compare the content of oxalates and cyanogenetic glucosides between raw and cooked tender stems. The plant collection and identification, phytochemical evaluation: phytochemical screening, preliminary (qualitative) analyses and in vitro assays. Phytochemical screening was performed by qualitative methods. The estimation of the content of secondary metabolites was evaluated by spectrophotometry-UV. Antioxidant activity was evaluated using the ABTS and DPPH assays and preliminary composition by the gravimetric method. The results obtained show that the stems of Dioscorea praehensilis are devoid of certain important chemical groups, the flavonoids were not detected and they were rich in total polyphenols (17.22 ± 0.16), tannins (19.32 ± 0.52) and anthocyanins (25.22 ± 0.04). Our extracts showed a lower antioxidant activity than that of positive controls. The samples are rich in carbohydrates and fiber, with low levels of proteins, lipids and ash. Dioscorea praehensilis has a high toxicity in HCN, but after a good cooking of about 1 hour, 99.97% of the cyanide are eliminated and does not have many oxalates. The results obtained show that Dioscorea praehensilis has a high dietary value and can therefore be used as a nutritive food.

Keywords:

Oxalate; Total cyanide; Anti-nutritional factors; D. praehensilis; Anthocyanes dosage

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Ngelinkoto, P. M., Lokassa, A.-M. K., Bulumuka, B., Maliani, J. K., Ngondo, M. M., Mbuli, R. L., Mukoko, J. B., & Mukeba, F. B. (2021). Phytochemical Study and Anti-nutritional Factors in Stems of Dioscorea praehensilis Benth (Dioscoreaceae). Journal of Botanical Research, 3(4), 1–10. https://doi.org/10.30564/jbr.v3i4.3455

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Vol. 3 , Iss. 4 (October 2021)

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