Phytochemical Study and Anti-nutritional Factors in Stems of Dioscorea praehensilis Benth (Dioscoreaceae)

Authors

  • Patience Mpia Ngelinkoto Department of chemistry and environment, Faculty of Sciences, National Pedagogic University, Kinshasa, Democratic Republic of Congo
  • André-Marie Kassia Lokassa Department of chemistry and environment, Faculty of Sciences, National Pedagogic University, Kinshasa, Democratic Republic of Congo
  • Bernadin Bulumuka Department of chemistry and environment, Faculty of Sciences, National Pedagogic University, Kinshasa, Democratic Republic of Congo
  • Jeff Kawaya Maliani Department of chemistry and environment, Faculty of Sciences, National Pedagogic University, Kinshasa, Democratic Republic of Congo
  • Myriam Mukadi Ngondo Department of Biology, Faculty of Sciences, National Pedagogic University, Kinshasa, Democratic Republic of Congo
  • Ruth Luntadila Mbuli Catholic University of the Congo, Kinshasa, Democratic Republic of Congo
  • Johnny Bopopi Mukoko Department of Biology, Faculty of Sciences, National Pedagogic University, Kinshasa, Democratic Republic of Congo
  • Florent Biduaya Mukeba Department of Biology, Faculty of Sciences, National Pedagogic University, Kinshasa, Democratic Republic of Congo

DOI:

https://doi.org/10.30564/jbr.v3i4.3455

Abstract

The aim of this research was to find and assay phytochemical compounds and various biological macromolecules of the tender stems of Dioscorea praehensilis benth and evaluate their antioxidant activity and to compare the content of oxalates and cyanogenetic glucosides between raw and cooked tender stems. The plant collection and identification, phytochemical evaluation: phytochemical screening, preliminary (qualitative) analyses and in vitro assays. Phytochemical screening was performed by qualitative methods. The estimation of the content of secondary metabolites was evaluated by spectrophotometry-UV. Antioxidant activity was evaluated using the ABTS and DPPH assays and preliminary composition by the gravimetric method. The results obtained show that the stems of Dioscorea praehensilis are devoid of certain important chemical groups, the flavonoids were not detected and they were rich in total polyphenols (17.22 ± 0.16), tannins (19.32 ± 0.52) and anthocyanins (25.22 ± 0.04). Our extracts showed a lower antioxidant activity than that of positive controls. The samples are rich in carbohydrates and fiber, with low levels of proteins, lipids and ash. Dioscorea praehensilis has a high toxicity in HCN, but after a good cooking of about 1 hour, 99.97% of the cyanide are eliminated and does not have many oxalates. The results obtained show that Dioscorea praehensilis has a high dietary value and can therefore be used as a nutritive food.

Keywords:

Oxalate, Total cyanide, Anti-nutritional factors, D. praehensilis, Anthocyanes dosage

References

[1] Bayiha, G., Temple, L., Mathe, S., Nesme, T. (2019). Typology and evolution perspective of organic farming in Cameroon. Agriculture Notebooks, 28, 3. https://doi.org/10.1051/cagri/2019003.

[2] FAO (2010). Develop greener cities in the Democratic Republic of Congo. FAO Urban and Peri-Urban Horticultural Program. 00153 Rome, Italy. 35 p.

[3] Minengu, J.D.D., Ikonso, M., Mawikiya, M. (2018). Family farming in the peri-urban areas of Kinshasa: analysis, challenges and perspectives (Bibliographical summary). African Journal of Environment and Agriculture, 1(1), 60-69.

[4] Asiedu, R., Sartie, A. (2010). Crops that feed the World 1. Yams Yams for income and food security. Food Security, 2: 305-315.

[5] Sesay, L., Norman, P.E., Massaquoi, A., Kobba, F., Allieu, A.P., Gboku, M.L., Fomba, S.N. (2013). Assessment of farmers’ indigenous knowledge and selection criteria of yam in Sierra Leone. Sky Journal of Agricultural Research, 2(1): 1 - 6.

[6] Adeniyan, O.N., Owolade, O.F. (2012). Comparative performance of improved white yam (Dioscorea rotundata) genotypes in the rainforest belt of Southwest Nigeria. International Research Journal of Agricultural Science and Soil Science, 2(4): 127-132.

[7] Dansi, A., Orobiyi, M., Dansi, P., Assogba, A., Sanni., Akpagana, K. (2013). Selection of sites for the in situ conservation of wild yams related to cultivated yams: case of Dioscorea praehensilis in Benin. International Journal of Biological and Chemical Sciences, 7(1): 60-74.

[8] Dumont, R., Dansi, A., Vernier, Ph., Zoundjihékpon, J. (2005). Biodiversity and domestication of yams in West Africa: Traditional practices leading to Dioscorea rotundata, 119 p.

[9] Chaïr, H., Perrier, X., Agbangla, C., Marchand J.L., Dainou, O., Noyer, J.L. (2005). Use of cpSSRs for the characterisation of yam phylogeny in Benin. Genome, 48: 674- 684.

[10] Mignouna, H.D., Dansi, A. (2003). Yam (Dioscorea sp.) domestication by the Nago and Fon ethnic groups in Benin. Genetic Resources and Crop Evolution 50(5): 519-528.

[11] Scarcelli, N., Tostain, S., Mariac, C., Agbangla, C., Daïnou, O., Berthaud, J., Pham, J-L. (2006). Genetic nature of yams (Dioscorea sp.) domesticated by famers in Benin (West Africa). Genetic Resources and Crop Evolution, 53(1): 121-130.

[12] Tamiru, M., Becker, C.H., Maass, B.L. (2008). Diversity, distribution and management of yam landraces (Dioscorea spp.) in Southern Ethiopia. Genetic Resources and Crop Evolution, 55(1): 115- 131.

[13] Ortiz, P.L., Berjano, R., Talavera, M., Arista, M. (2009). The role of resources and architecture in modeling floral variability for the monoecious amphicarpic emex spinosa (Polygonaceae). American Journal of Botany, 96(11): 2062-2073.

[14] Maxted, N., White, K., Valkoun, J., Konopka, J., Hargreaves, S. (2008). Towards a conservation strategy for Aegilops species. Plant Genetic Resources: Characterization and Utilization, 6(2):126-141.

[15] Chatzav, M., Peleg, Z., Ozturk, L., Yazici, A., Fahima, T., Cakmak, I., Saranga, Y. (2010). Genetic diversity for grain nutrients in wild emmer wheat: potential for wheat improvement. Annals of Botany, 105 (7):1211- 1220.

[16] Luse, R.A. (1975). The Role of Grain Legume in Tropical Nutrition, Proc. 1st African Nutrition Congress.

[17] Bruneton, J. (1999). Pharmacognosy, Phytochemistry and Medicinal Plants. Technical Edition and Documentation-Lavoisier. Paris 3rd edition, 421- 499.

[18] Harborne, JB, Baxter, H. (1995). Phytochemical dictionary: a handbook of bioactive compounds from plants. 4 John St, Taylor & Francis Ltd, London.

[19] Kokate, CK, Purohit, AP, Gokhale, SB. (1981). Test book of Pharmacognosy. Nirali Prakashan, Pune, 2003.

[20] Gutfinger, T. Polyphenols in olive oils. J Am Oil Chem Soc. 58:966-968.

[21] Querttier-Deleu, C., Gressier, B., Vassseur, J., Dine, T., Brunet, C., Luyckx, M.C., Cazin, J.C., Bailleu, F., Trotin, F. (2000). Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. J Ethnopharmacol. 72:35-42.

[22] Zhang, S.Y., Zheng, C.G., Yan, X.Y., Tian, W.X. (2008). Low concentration of condensed tannins from catechu significantly inhibits fatty acid synthase and growth of MCF-7 cells. Biochem Biophys Com. v371:654-658.

[23] Sun, B., Ricardo-da-Silva, J.M., Spranger. (1998). Critical factors of vanillin assay for catechins and proanthocyanidins. J Agric Food Chem. 46(10):42674274.

[24] Adedapo, A.A., Jimoh, F.O., Koduru, S. et al. (2008). Antibacterial and antioxidant properties of the methanol extracts of the leaves and stems of Calpurnia aurea. BMC Complement Altern Med 8, 53. https://doi.org/10.1186/1472-6882-8-53.

[25] Mukeba, F.B., Bopopi, J.M., Mayangi, M.M., Kikweta, C., Bamba, J.V., Kitwa, F., Mutwale, P.K., Ngbolua, K.J.P., Ngombe, N.K., Mpiana, P.T., Mbemba, T.F. (2020). Selenium content, antibacterial, antioxidant and anti-sickling activities of Zanthoxylum gilletii (De Wild) P.G. Waterman (Rutaceae). Discovery Phytomedicine 7(4): 145-154. DOI: https://doi.org/10.15562/phytomedicine.2020.141.

[26] Mbemba, F., Remacle, J. (1992). Inventory and chemical composition of traditional foods and foodstuffs from Kwango-Kwilu in Zaire. Namur University Press. Namur, p : 80.

[27] Degroote. (1965). Analyzes of macromolecules by physico-chemical / phytochemical methods. 1964, p 66-72.

[28] Vervack, W. (1982). Food analysis. Laboratory of Biochemistry of Nutrition. Catholic University of Louvain-la-Neuve / Belgium. 2nd Part. pp.10-45.

[29] AOAC. (1970). Official methods of analysis of the association of official analytical chemists, 11th Ed. Washington DC.

[30] Sadasivam, S., Manickam, A. (1996). Biochemical methods for agricultural science. Wiley Eastern Limited, New Delhi.18.

[31] Mbemba, F. (2013). Traditional foods and foodstuffs from Bandundu in DR Congo. Ed. L’Harmattan, Paris. French.

[32] Makengo, K.G., Bongeli, A., Mbemba, F.T. (2015). Contribution to the study of the biological value of a complementary food based on local food resources: Case of Vamine. Ann Fac. Sci (University of Kinshasa). 1:43-51. French.

[33] AOAC. (1984). Official methods of analysis (p. 500, 14th ed.). Arlington, VA, USA: Association of Official Analytical Chemists.

[34] Oke, O.L. (1966). Chemical studies on some Nigerian Vegetables. Trop. Sci. Trop. Sci. 8(3): 128-132.

[35] Bukatuka, F.C., Ngombe, K.N., Mutwale, K.P., Moni, B.M., Makengo, K.G., Pambu, L.A., Bongo, N.G., Mbombo M.P., et al. (2016). Bioactivity and Nutritional Values of Some Dioscorea Species Traditionally Used as Medicinal Foods in Bandundu, DR Congo. European Journal of Medicinal Plants 14(1): 1-11, Article no.EJMP.25124. ISSN: 2231-0894, NLM ID: 101583475.

[36] Sherman, H.C. (1952). Chemistry of Food and Nutrition. The MacMilan Company, New York.

[37] Bray, T.M, Taylor CG. (1993). Tissue glutathione, nutrition, and oxidative stress. Can J Physiol Pharmacol, 71 and 746-5.

[38] AOAC. (1980). Anonymous Official methods of analysis, Association of Official Analytical Chemist, Washington DC, 10th Edition.

[39] Hossain, M.A., Nagooru, M.R. (2011). Biochemical profiling and total flavonoids and contents of leaves crude extract of endemic medicinal plants Corydyline terminalis L Kunth. Pharmacognosy Journal, 3(24): 25-29.

[40] Suresh, S.N., Nagarajan, N. (2009). Preliminary phytochemical and antimicrobial activity analysis of Begonia malabarica Lam. J. Basic appl. biol, 3(1&2): 59-61.

[41] Saidu, A.N., Mann, A., Onuegbu, C.D. (2012). Pytochemical screening and hypoglycemic effect of aqueous Blighia sapida root bark extract on normoglycemic albino rats. Bri J Pharmaceu Res, 2: 89-97.

[42] Sasikumar, J.M., Maheshu, V., Aseervatham, G.S.B., Darsini, D.T.P. (2010). In vitro antioxidant activity of Hedyotis corymbosa (L) Lam aerial plants. Indian J Biochem and Biophy; 47: 49-52.

[43] Bhandari, M.R., Kawabata, J. (2004). Organic acid, phenolic content and antioxidant activity of wild yam (Dioscorea spp.) tubers of Nepal. Food Chemistry. 88(2):163168.

[44] Subasini, U., Thenmozhi, S., Sathyamurthy, D., Vetriselvan, S., Rajamanickam, G.V., Dubey, G.P. (2013). Pharmacognostic and phytochemical investigations of Dioscorea bulbifera L Int. J. of Pharm. & Life Sci. (IJPLS), 4(5): 2693-2700.

[45] Floegel, A., Kim, D.O., Chung, S.J., Koo, S.I., Chun, O.K. (2011). Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. J Food Comp Anal. 24(7):10431048.

[46] Ngbolua, K.N., Bishola, T.T., Mpiana, P.T., Mudogo, V., Tshibangu, D.S.T., Ngombe, K.N., Tshilanda, D.D., Baholy, R. (2014a). In vitro antisickling and free radical scavenging activities of Pentaclethra macrophylla Benth (Fabaceae). J Advancement Med Life Sci. V1I2. DOI: https://doi.org/10.15297/JALS.V1I2.03.

[47] Ngbolua, K.N., Bishola, T.T., Mpiana, P.T., Mudogo, V., Tshibangu, D.S.T., Ngombe, K.N., Ekutsu, E.G., Gbolo, B.Z., Kabena, N.O. (2014b). Ethno-pharmacological survey,in vitro antisickling and free radical scavenging activities of Carapa procera DC. Stem bark (Meliaceae). Nova J Med Biol Sci. 2(2):01-14.

[48] Ngbolua, K.N., Bishola, T.T., Mpiana, P.T., Mudogo, V., Tshibangu, D.S.T., Ngombe K.N., Ekutsu, E.G., Tshilanda, D.D., Gbolo, B.Z., Mwanangombo, D.T., Fatiany, P.R., Baholy, R. (2014c). Ethno-botanical survey, in vitro antisickling and free radical scavenging activities of Garcinia punctata Oliv. (Clusiaceae). J Adv Bot Zool.; V1I2. DOI: https://doi.org/10.15297/JABZ.V1I2.04.

[49] Ngbolua, K.N., Mubindukila, N., Mpiana, P.T., Tshibangu, D.S.T., Masengo, C.A., Nzongola, K., Baholy, R., Fatiany, P.R. (2014d). Phytochemical screening, antibacterial and antioxidant activities of Anthocleista liebrechtsiana Wild & T. Durand (Gentianaceae) originated from Democratic Republic of the Congo. J Adv Med Life Sci.V1I3. DOI: https://doi.org/10.15297/JALS.V1I3.04.

[50] Savage, M. (2000). Effect of cooking on the soluble and insoluble oxalate content of some New Zealand foods. J. Food Compos. Anal. 13(3): 201-206.

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Ngelinkoto, P. M., Lokassa, A.-M. K., Bulumuka, B., Maliani, J. K., Ngondo, M. M., Mbuli, R. L., Mukoko, J. B., & Mukeba, F. B. (2021). Phytochemical Study and Anti-nutritional Factors in Stems of Dioscorea praehensilis Benth (Dioscoreaceae). Journal of Botanical Research, 3(4), 1–10. https://doi.org/10.30564/jbr.v3i4.3455

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Vol. 3 , Iss. 4 (October 2021)

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