Comparative Toxicity of Neem and Peppermint Oils Nano Formulations against Agrotis ipsilon (Hufn.) Larvae (Lepidoptera: Noctuidae)
Applications of nanotechnology in agriculture will result in the development of efficient and potential approaches towards the management of insect pests. The toxicity effects of four essential oils peppermint, thyme, camphor and sage oils were tested against the fourth instar larvae of Agrotis ipsilon to select the most effective essential oil to be converted to the nano form. According to the results obtained, peppermint oil was the most toxic compound, which has been used in the present investigation compared with neem oil. The toxicity of bulk and nano- formulations of neem and pepper mint oils were tested against 2nd and 4th instar larvae of A. ipsilon under laboratory conditions of 25±2 °C& 65 -70 % R.H.relative humidity The results show that the LC50 value (the concentration used which kill 50% of the tested individuals)of loaded neem or pepper mint were lower (0.62 and 36.47 ppm) compared with neem or pepper mint oil nano-emulsion and bulk neem for the second larval instar. The different formulations of neem are more potent than in case of peppermint oil, as LC50 and LC90 values were significantly lower.The same trend was found concerning the 4th larval instar. Age of treated larvae had a detrimental effect on the response to the compounds tested. It was noticed that the younger larvae were much more sensitive to the prepared compounds compared to the older ones. The least LC50 value for loaded neem nano-emulsion was 6.68 ppm compared with the highest value for bulk neem oil (16.68 ppm ). Also, LC90 values followed the same trend as in case ofLC50. Again, the toxicity of loaded peppermint oil had the most insecticidal activity as expressed by the lowest LC50 value (51.9 ppm) with more insecticidal effect than the bulk(125.43 ppm) or nano-emulsion (85.43 ppm). The present results indicated that these novel systems could be used in integrated pest management program for A. ipsilon control.
Keywords:Agrotis ipsilon larvae, Toxicity, Neem, Pepper mint oil, Nano formulations
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