Improved Heat FT Induction Leads to Earlier and More Prolific Flowering in Poplar

Authors

  • Abdul Azeez School of Forest Resources and Environmental Science, Michigan Technological University, Houghton, MI 49931, United States
  • Victor Busov School of Forest Resources and Environmental Science, Michigan Technological University, Houghton, MI 49931, United States

DOI:

https://doi.org/10.30564/jrb.v1i2.1013

Abstract

Trees have a long juvenile phase before reproductive onset. This makes their breeding and studying floral development difficult. Precocious flowering using FT technology has shown promise. However, transgenic FT overexpression has significant negative pleiotropic effects. Hence, there has been interest in inducible FT expression for flower induction. Previously reported heat inducible expression of FT in poplar successfully induced flowering. However, flowering was sporadic and took up to 6 weeks. Here we report improvements in the protocol, which led to faster and more prolific flowering. Specifically, we increased the once to three times daily heat treatment. The repeated heat inductive treatments led to nearly five times higher FT expression, compared to the single daily treatment. The highly increased FT expression led to significant acceleration and abundance of flowering.

Keywords:

Poplar; Heat shock promoter; Flowering Locus T; Flowering; Heat induction

References

[1] Flachowsky, H., et al., A review on transgenic approaches to accelerate breeding of woody plants. 2009, 128(3): 217-226. DOI: https://doi.org/10.1111/j.1439-0523.2008.01591.x

[2] Meilan, R.J.N.F., Floral induction in woody angiosperms. 1997, 14(3): 179-202. DOI: https://doi.org/10.1023/a:1006560603966

[3] Weigel, D. and O. Nilsson, A developmental switch sufficient for flower initiation in diverse plants. Nature, 1995, 377(6549): 495-500. DOI: https://doi.org/10.1038/377495a0

[4] Klocko, A.L., et al., FT overexpression induces precocious flowering and normal reproductive development in Eucalyptus. Plant Biotechnology Journal, 2016, 14(2): 808-819. DOI: https://doi.org/10.1111/pbi.12431

[5] Tang, M., et al., An ortholog of LEAFY in Jatropha curcas regulates flowering time and floral organ development. Sci Rep, 2016, 6: 37306. DOI: https://doi.org/10.1038/srep37306

[6] Borghi, L., Inducible gene expression systems for plants. Methods Mol Biol, 2010, 655: 65-75. DOI: https://doi.org/10.1007/978-1-60761-765-5_5

[7] Zhang, H., et al., Precocious flowering in trees: the FLOWERING LOCUS T gene as a research and breeding tool in Populus. J Exp Bot, 2010, 61(10): 2549-60. DOI: https://doi.org/10.1093/jxb/erq092

[8] Vandesompele, J., et al., Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biology, 2002, 3(7): research0034.1. DOI: https://doi.org/10.1186/gb-2002-3-7-research0034. DOI: http://doi.org/10.30564/jrb.v1i2.1013

[9] Azeez, A., et al., A tree ortholog of APETALA1 mediates photoperiodic control of seasonal growth. Curr Biol, 2014, 24(7): 717-24. DOI: https://doi.org/10.1016/j.cub.2014.02.037

[10] Kardailsky, I., et al., Activation tagging of the floral inducer FT. Science, 1999, 286(5446): 1962-5. DOI: https://doi.org/10.1126/science.286.5446.1962

[11] Kobayashi, Y., et al., A pair of related genes with antagonistic roles in mediating flowering signals. Science, 1999, 286(5446): 1960-2. DOI: https://doi.org/10.1126/science.286.5446.1960

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How to Cite

Azeez, A., & Busov, V. (2019). Improved Heat FT Induction Leads to Earlier and More Prolific Flowering in Poplar. Journal of Botanical Research, 1(2), 15–18. https://doi.org/10.30564/jrb.v1i2.1013

Issue

Vol. 1 , Iss. 2 (July 2019)

Article Type

Article

License

Copyright © 2019 Author(s)

Creative Commons License

This is an open access article under the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) License.

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