Effects of Arbuscular Mycorrhizal Fungi on the Physiology and Saponin Synthesis of Paris polyphylla var. yunnanensis at Different Nitrogen Levels

Authors

  • Can Huang

    College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan, 650201, China

    Guangxi Subtropical Crops Research Institute, Nanning, Guangxi, 530001, China
  • Shubiao Qian

    College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan, 650201, China
  • Xiaoxian Li

    Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, 650201, China
  • Xiahong He

    College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan, 650201, China
  • Shuhui Zi

    College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan, 650201, China
    State Key Laboratory of Conservation and Utilization of Bio-resources in Yunnan, National & Local Joint Engineering Research Center on Gemplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest China, Yunnan Agricultural University, Kunming, Yunnan, 650500, China
  • Congfang Xi

    College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan, 650201, China
  • Rui Shi

    Southwest Forestry University, Kunming, Yunnan, 650224, China
  • Tao Liu

    College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan, 650201, China

DOI:

https://doi.org/10.30564/jbr.v5i3.5518
Received: 7 March 2023 | Revised: 6 May 2023 | Accepted: 6 May 2023 | Published Online: 26 May 2023

Abstract

Arbuscular mycorrhizal fungi (AMF) are important members of the plant microbiome and affect the uptake and transfer of mineral elements by forming a symbiotic relationship with plant roots. Nitrogen (N), as an important mineral element, can directly affect plant growth and development at different N levels. It has been confirmed that inoculation with AMF can improve the efficiency of N utilization by plants. However, there are still fewer reports on the dynamic relationship between arbuscular mycorrhizal and plant secondary metabolites at different nitrogen levels. In this experiment, the physiological indexes and genes related to saponin synthesis were determined by applying different concentration gradients of nitrogen to the medicinal plant P. polyphylla var. yunnanensis infested by AMF as the test material. It was found that nitrogen addition increased the biomass, chlorophyll content, and nutrient content of above- and below-ground plant parts and increased the content of saponin content of P. polyphylla var. yunnanensis to some extent, but AMF inoculation increased the saponin content of P. polyphylla var. yunnanensis more significantly. AMF inoculation also promoted the expression of genes related to the saponin synthesis pathway, including 3-hydroxy-3-methylglutaryl coenzyme A synthase (HMGS), squalene epoxidase 1 (SE1), and cycloartenol synthase (CAS), which is in according with the accumulation of saponin in plants. It also may increase the saponin content of AMF plants by altering the expression of P450s and UGTs related to saponin synthesis.

Keywords:

Nitrogen; Arbuscular mycorrhizal fungi; Saponin; P. polyphylla var. yunnanensis

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Huang, C., Qian, S., Li, X., He, X., Zi , S., Xi, C., Shi, R., & Liu, T. (2023). Effects of Arbuscular Mycorrhizal Fungi on the Physiology and Saponin Synthesis of Paris polyphylla var. yunnanensis at Different Nitrogen Levels. Journal of Botanical Research, 5(3), 1–26. https://doi.org/10.30564/jbr.v5i3.5518

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Vol. 5 , Iss. 3 (July 2023)

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