Effects of Exogenous Calcium on Datura Seed Germination under Drought Stress

Authors

  • Kaiyu Qin Shanxi Normal University, Linfen, Shanxi, 041004, China
  • Shuaiqun Fan Shanxi Normal University, Linfen, Shanxi, 041004, China
  • Fenguo Zhang Shanxi Normal University, Linfen, Shanxi, 041004, China
  • Yongji Wang Shanxi Normal University, Linfen, Shanxi, 041004, China

DOI:

https://doi.org/10.30564/jrb.v1i2.862

Abstract

With polyethylene glycol (PEG-6000), of 0% (CK), 5%, 10%, 15%, 25% used to simulate drought stress, and CaCl2 concentration 0 (CK), of 15, 20, 25 and 30mmol/L as ion gradient of exogenous calcium, the effects of drought, exogenous calcium and the interaction between the two on the Datura seed germination, so as to explore the optimal application amount of exogenous calcium to ease the suppression of drought stress on Datura seed germination. The results showed that the germination rate, germination potential and germination index of the Datura seeds were significantly lower than those of the control group. Under the normal moisture condition, exogenous calcium of moderate and low concentration had no significant effect on the Datura seed germination, while that of high concentration showed an inhibitory effect on the seed germination. Under drought stress, with the increasing concentration of exogenous calcium, the three indicators of Datura seeds showed a trend of increasing first and then decreasing. When the exogenous calcium had the concentration of 20 mmol/L, all the indicators of seed germination reached the maximum value, while showed a downward trend when exogenous calcium concentration was 25-30 mmol/L, and even increasingly sharp with drought intensifying. Therefore, in the production and utilization of Datura, 20 mmol/L of exogenous calcium can be used to soak seeds before sowing to improve the emergence rate under low and moderate drought conditions.

Keywords:

Datura, Drought stress, Exogenous calcium, Seed germination

References

[1] Wang, Y.J., Wang, J.J., Lai, L.M., Jiang, L.H., Zhuang, P., Zhang, L.H., Zheng, Y.R., Baskin, J.M. & Baskin, C.C.. Geographic variation in seed traits within and among forty-two species of Rhododendron (Ericaceae) on the Tibetan plateau: relationships with altitude, habitat, plant height, and phylogeny. Ecology and Evolution, 2014b, 4: 1913-1923.

[2] Baskin, J.M. & Baskin, C.C.. Pollen limitation and its effect on seed germination. Seed Science Research, 2018, 28: 253-260.

[3] Wang, Y.J., Lai, L.M., Du, H., Jiang, L.H., Wang, F., Zhang, C., Zhuang, P. & Zheng, Y.R.. Phylogeny, habitat together with biological and ecological factors can influence germination of 36 subalpine Rhododendron species from the eastern Tibetan Plateau. Ecology and Evolution, 2018, 8: 3589-3598.

[4] Dong, L.L., Zhang, H.D., Wang, L.Q., Yu, D.S., Yang, F.X., Shi, X.Z., Saleem, H. & Akhtar, M.S.. Irrigation with sediment-laden river water affects the soil texture and composition of organic matter fractions in arid and semi-arid areas of Northwest China. Geoderma, 2018, 328: 10-19.

[5] Zhu, H.X., Fu, B.J., Lv, N., Wang, S. & Hou, J.. Multivariate control of root biomass in a semi-arid grassland on the Loess Plateau, China. Plant and Soil, 2014, 379: 315-324.

[6] Cui, Y.X., Fang, L.C., Guo, X.B., Wang, X., Zhang, Y.J., Li, P.F. & Zhang, X.C.. Ecoenzymatic stoichiometry and microbial nutrient limitation in rhizosphere soil in the arid area of the northern Loess Plateau, China. Soil Biology & Biochemistry, 2018, 116: 11-21.

[7] Yu, T.F., Feng, Q., Si, J.H., Xi, H.Y., Li, Z.X. & Chen, A.F.. Hydraulic redistribution of soil water by roots of two desert riparian phreatophytes in northwest China's extremely arid region. Plant and Soil, 2013, 372: 297-308.

[8] Zhang, S.P., Shao, M.G. & Li, D.F.. Prediction of soil moisture scarcity using sequential Gaussian simulation in an arid region of China. Geoderma, 2017, 295: 119-128.

[9] Jiang, A.W., Jiang, D.M., Goodale, E. & Wen, Y.G.. Nest predation on birds that nest in rock cavities in a tropical limestone forest of southern China. Global Ecology and Conservation, 2017, 10: 154-158.

[10] Gao, L., Miao, Z.W., Bai, Z.K., Zhou, X.Y., Zhao, J.K. & Zhu, Y.M.. A case study of ecological restoration at the Xiaoyi Bauxite Mine, Shanxi Province, China. Ecological Engineering, 1998, 11: 221-229.

[11] Miao, Z. & Marrs, R.. Ecological restoration and land reclamation in open-cast mines in Shanxi Province, China. Journal of Environmental Management, 2000, 59: 205-215.

[12] Shi, Z.L., Wen, A.B., Walling, D.E., Wang, Y.Y. & Chen, J.C.. Exploring particle size selectivity effects during erosion of purple soils in Chongqing municipality, China. Journal of Soils and Sediments, 2017, 17: 1191-1196.

[13] Xi, J., Zhao, X., Wang, X. & Zhang, Z.. Assessing the impact of land use change on soil erosion on the Loess Plateau of China from the end of the 1980s to 2010. Journal of Soil and Water Conservation, 2017, 72: 452-462.

[14] Yu, K.K., Xu, H., Lan, J.H., Sheng, E.G., Liu, B., Wu, H.X., Tan, L.C. & Yeager, K.M.. Climate change and soil erosion in a small alpine lake basin on the Loess Plateau, China. Earth Surface Processes and Landforms, 2017, 42: 1238-1247.

[15] McManamen, C., Nelson, C.R. & Wagner, V.. Timing of seeding after herbicide application influences rates of germination and seedling biomass of native plants used for grassland restoration. Restoration Ecology, 2018, 26: 1137-1148.

[16] Zajc, M., Gosar, A. & Celarc, B.. GPR Study of a Thrust-Fault in an Active Limestone Quarry (SW Slovenia). Journal of Environmental and Engineering Geophysics, 2018, 23: 457-468.

[17] Zhang, S., Lu, P., Cantrell, D., Zaretskiy, Y., Jobe, D. & Agar, S.M.. Improved quantification of the porosity-permeability relationship of limestones using petrographical texture. Petroleum Geoscience, 2018, 24: 440-448.

[18] Birnbaum, C., Bradshaw, L.E., Ruthrof, K.X. & Fontaine, J.B.. Topsoil Stockpiling in Restoration: Impact of Storage Time on Plant Growth and Symbiotic Soil Biota. Ecological Restoration, 2017, 35: 237-245.

[19] Moreno, M., de-Bashan, L.E., Hernandez, J.P., Lopez, B.R. & Bashan, Y.. Success of long-term restoration of degraded arid land using native trees planted 11 years earlier. Plant and Soil, 2017, 421: 83-92.

[20] Wang, F.M., Ding, Y.Z., Sayer, E.J., Li, Q.L., Zou, B., Mo, Q.F., Li, Y.W., Lu, X.L., Tang, J.W., Zhu, W.X. & Li, Z.A.. Tropical forest restoration: Fast resilience of plant biomass contrasts with slow recovery of stable soil C stocks. Functional Ecology, 2017, 31: 2344-2355.

[21] Koziol, L., Schultz, P.A., House, G.L., Bauer, J.T., Middleton, E.L. & Bever, J.D.. The Plant Microbiome and Native Plant Restoration: The Example of Native Mycorrhizal Fungi. Bioscience, 2018, 68: 996-1006.

[22] van Zuidam, J.P., van Leeuwen, C.H.A., Bakker, E.S., Verhoeven, J.T.A., Ijff, S., Peeters, E.T.H.M., van Zuidam, B.G. & Soons, M.B.. Plant functional diversity and nutrient availability can improve restoration of floating fens via facilitation, complementarity and selection effects. Journal of Applied Ecology, 2019, 56: 235-245.

[23] Loddo, D., Sousa, E., Masin, R., Calha, I.M., Zanin, G., Fernandez-Quintanilla, C. & Dorado, J.. Germination response of local Southern European populations of Datura stramonium at a range of constant temperatures. Weed Research, 2014, 54: 356-365.

[24] Camargo, I.D., Nattero, J., Careaga, S.A. & Nunez-Farfan, J.. Flower-level developmental plasticity to nutrient availability in Datura stramonium: implications for the mating system. Annals of Botany, 2017, 120: 603-615.

[25] Cisneros-Silva, A., Castillo, G., Chavez-Pesqueira, M., Bello-Bedoy, R., Camargo, I.D. & Nunez-Farfan, J.. Light limitation reduces tolerance to leaf damage in Datura stramonium. Evolutionary Ecology Research, 2017, 18: 351-362.

[26] Jimenez-Lobato, V., Martinez-Borda, E., Nunez-Farfan, J., Valverde, P.L., Cruz, L.L., Lopez-Velazquez, A., Santos-Gally, R. & Arroyo, J.. Changes in floral biology and inbreeding depression in native and invaded regions of Datura stramonium. Plant Biology, 2018, 20: 214-223.

[27] Andrews, A.E.. The active constituents of the Indian solanaceous plants Datura stramonium, D fastuosa, and D Metel. Journal of the Chemical Society, 1911, 99: 1871-1877.

[28] Ashford, D., Desai, N.N., Allen, A.K., Neuberger, A., Oneill, M.A. & Selvendran, R.R.. Structural Studies of the Carbohydrate Moieties of Lectins from Potato (Solanum-Tuberosum) Tubers and Thornapple (Datura-Stramonium) Seeds. Biochemical Journal, 1982, 201: 199-208.

[29] Boyette, C.D. & Turfitt, L.B.. Factors Influencing Biocontrol of Jimsonweed (Datura-Stramonium L) with the Leaf-Spotting Fungus Alternaria-Crassa. Plant Science, 1988, 56: 261-264.

[30] Arouko, H., Matray, M.D., Braganca, C., Mpaka, J.P., Chinello, L., Castaing, F., Bartou, C. & Poisot, D.. Voluntary poisoning by ingestion of Datura stramonium. Another cause of hospitalization in youth seeking strong sensations. Annales De Medecine Interne, 2003, 154: S46-S50.

[31] Lewis, O.A.M. & Probyn, T.A.. N-15 Incorporation and Glutamine-Synthetase Inhibition Studies of Nitrogen Assimilation in Leaves of the Nitrophile, Datura-Stramonium L. New Phytologist, 1978, 81: 519- 526.

[32] Baselga, J.M., Pigrau, C. & Martinezvazquez, J.M.. Datura-Stramonium - a Flourishing Old Hallucinogenic Agent. Medicina Clinica, 1985, 84: 715-715.

[33] Ballica, R., Ryu, D.D.Y. & Kado, C.I.. Tropane Alkaloid Production in Datura-Stramonium Suspension - Cultures - Elicitor and Precursor Effects. Biotechnology and Bioengineering, 1993, 41: 1075-1081.

[34] Matsuda, K., Aoki, J., Uchida, M.K. & Suzukinishimura, T.. Datura-Stramonium Agglutinin Released Histamine from Rat Peritoneal Mast-Cells That Was Inhibited by Pertussis Toxin, Haptenic Sugar and N - Acetylglucosamine - Specific Lectins - Involvement of Glycoproteins with N-Acetylglucosamine Residues. Japanese Journal of Pharmacology, 1994, 66: 195-204.

[35] Khan, A.A. & Karssen, C.M.. Photoreversible Changes during Germination Prohibitive Imbibition in Peg-6000 Solution Modulating Germination Responses of Chenopodium-Bonus-Henricus L Seeds. Plant Physiology, 1979, 63: 15-15.

[36] Masuda, M., Hata, N., Ombwara, F.K. & Agong, S.G.. Effects of acid scarification, priming with PEG, NaCl or sea water as osmoticum and dehydration on spinach seed germination at 30 degrees C. Journal of the Japanese Society for Horticultural Science, 2005, 74: 134-138.

[37] Anil, V.S., Harmon, A.C. & Rao, K.S.. Spatio-temporal accumulation and activity of calcium-dependent protein kinases during embryogenesis, seed development, and germination in sandalwood. Plant Physiology, 2000, 122: 1035-1043.

[38] Bonilla, I., El-Hamdaoui, A. & Bolanos, L.. Boron and calcium increase Pisum sativum seed germination and seedling development under salt stress. Plant and Soil, 2004, 267: 97-107.

[39] Lucchese, J.R., Lazarotto, M., Fior, C.S., de Sa, L.C. & Brose, C.B.. Analysis of seed vigor and germination of Toona ciliata M. Roem. var. australis. Journal of Seed Science, 2018, 40: 388-395.

[40] Pierce, G.L., Warren, S.L., Mikkelsen, R.L. & Linker, H.M.. Effects of soil calcium and pH on seed germination and subsequent growth of large crabgrass (Digitaria sanguinalis). Weed Technology, 1999, 13: 421-424.

[41] Salahshoor, F. & Kazemi, F.. Effect of calcium on reducing salt stress in seed germination and early growth stage of Festuca ovina L. Plant Soil and Environment, 2016, 62: 460-466.

[42] Sharma, S.S., Sharma, S. & Rai, V.K.. The Effect of Egta, Calcium-Channel Blockers (Lanthanum Chloride and Nifedipine) and Their Interaction with Abscisic-Acid on Seed-Germination of Brassica-Juncea Cv Rlm-198. Annals of Botany, 1992, 70: 295-299.

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How to Cite

Qin, K., Fan, S., Zhang, F., & Wang, Y. (2019). Effects of Exogenous Calcium on Datura Seed Germination under Drought Stress. Journal of Botanical Research, 1(2), 8–14. https://doi.org/10.30564/jrb.v1i2.862

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